Transforming growth factor beta activity in urine of patients with type 2 diabetes and diabetic nephropathy.

Abstract

Diabetic nephropathy (DN) is characterized structurally by progressive mesangial deposition of extracellular matrix (ECM). Transforming growth factor-ss (TGF-ss) is considered to be one of the major cytokines involved in the regulation of ECM synthesis and degradation. Several studies suggest that an increase in urinary TGF-ss levels may reflect an enhanced production of this polypeptide by the kidney cells. We evaluated TGF-ss in occasional urine samples from 14 normal individuals and 23 patients with type 2 diabetes (13 with persistent proteinuria >500 mg/24 h, DN, 6 with microalbuminuria, DMMA, and 4 with normal urinary albumin excretion, DMN) by enzyme immunoassay. An increase in the rate of urinary TGF-ss excretion (pg/mg U Creat.) was observed in patients with DN (296.07 +/- 330.77) (P<0.001) compared to normal individuals (17.04 +/- 18.56) (Kruskal-Wallis nonparametric analysis of variance); however, this increase was not observed in patients with DMMA (25.13 +/- 11.30) or in DMN (18.16 +/- 11.82). There was a positive correlation between the rate of urinary TGF-ss excretion and proteinuria (r = 0.70, alpha = 0.05) (Pearson's analysis), one of the parameters of disease progression.

• Authors: E W Rivarola, M Moyses-Neto, M Dantas, C G Da-Silva, R Volpini, T M Coimbra
• Journal: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas (Braz J Med Biol Res) Vol. 32 Issue 12 Pg. 1525-8 (Dec 1999) ISSN: 0100-879X [Print] Brazil
• PMID: 10585634 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Review)

Chemical References

• Biomarkers
• Transforming Growth Factor beta

Topics

• Adult
• Biomarkers (urine)
• Diabetes Mellitus, Type 2 (urine)
• Diabetic Nephropathies (urine)
• Extracellular Matrix (metabolism)
• Humans
• Kidney (metabolism)
• Middle Aged
• Proteinuria (etiology)
• Transforming Growth Factor beta (urine)