alpha5 integrin distribution and TGFbeta1 gene expression in supraglottic carcinoma: their role in neoplastic local invasion and metastasis.

Abstract

BACKGROUND:

Head and neck carcinomas are characterized by tumor-infiltrating lymphocytes (TIL) producing cytokines. Adhesion molecules, extracellular matrix proteins (ECMPs), and cytokines regulate cell-cell and cell-ECMPs interactions. We investigated the distribution of these proteins to contribute to better understanding of their role in local tumor invasion and metastasis.

METHODS:

Distribution of integrins, laminin, type IV collagen, tenascin, and fibronectin was immunohistochemically evaluated in 13 supraglottis carcinomas. Cytokines gene expression was assessed by reverse-transcriptase-polymerase chain reaction (RT-PCR).

RESULTS:

Neoplastic cells were alpha2beta1, alpha3beta1, alpha4beta1, alpha5beta1 and alpha6beta1 positive. Normal and metaplastic epithelium was alpha5beta1 negative; the stroma of primary and metastatic tumors was tenascin and fibronectin positive. TGFbeta1 and IFNgamma gene expression was observed in the majority of tumors.

CONCLUSIONS:

Because TGFbeta1 is known to down-modulate immune processes and to increase alpha2beta1, alpha5beta1, and tenascin distribution, we propose that their expression in neoplastic cells of supraglottis carcinoma might represent an immune-related process able to help tumor growth and progression.

• Authors: D Vitolo, L Ciocci, P Ferrauti, E Cicerone, A Gallo, M De Vincentiis, C D Baroni
• Journal: Head & neck (Head Neck) Vol. 22 Issue 1 Pg. 48-56 (Jan 2000) ISSN: 1043-3074 [Print] United States
• PMID: 10585605 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2000 John Wiley & Sons, Inc. Head Neck 22: 48-56, 2000.

Chemical References

• Antigens, CD
• Biomarkers, Tumor
• Cell Adhesion Molecules
• Cytokines
• Extracellular Matrix Proteins
• Integrin alpha5
• Integrins
• Transforming Growth Factor beta

Topics

• Antigens, CD (genetics, metabolism)
• Base Sequence
• Biomarkers, Tumor (analysis)
• Carcinoma in Situ (genetics, pathology)
• Carcinoma, Squamous Cell (genetics, pathology, secondary)
• Cell Adhesion Molecules (metabolism)
• Culture Techniques
• Cytokines (genetics)
• Extracellular Matrix Proteins (metabolism)
• Female
• Gene Expression
• Humans
• Immunohistochemistry
• Integrin alpha5
• Integrins (metabolism)
• Laryngeal Neoplasms (genetics, pathology)
• Male
• Molecular Sequence Data
• Neoplasm Invasiveness
• Reference Values
• Reverse Transcriptase Polymerase Chain Reaction
• Sensitivity and Specificity
• Transforming Growth Factor beta (genetics, physiology)