Abstract | BACKGROUND: METHODS AND RESULTS: We have detected a repeat polymorphism in the promoter region of the human prostacyclin synthase gene. The number of 9-bp (CCGCCAGCC) repeats in the promoter region, which encodes a tandem repeat of Sp1 transcriptional binding sites, varied between 3 and 7 in Japanese subjects. Luciferase reporter analysis indicated that the alleles of 3 and 4 repeats (R3 and R4, respectively) had less promoter activity in cultured human umbilical vein endothelial cells. We then investigated the possible association of this repeat polymorphism with blood pressure in a large population-based sample (the Suita Study), which consisted of 4971 Japanese participants. Multivariate models indicated that participants with the R3R3, R3R4, or R4R4 genotype (SS genotype, n=80) had significantly higher systolic pressure (P=0.0133) and pulse pressure (P=0.0005). The odds ratio of hypertension (140/90 mm Hg) for the SS genotype was 1.942 (95% confidence interval 3.20 to 1.19, P=0.0084). CONCLUSIONS:
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Authors | N Iwai, T Katsuya, K Ishikawa, T Mannami, J Ogata, J Higaki, T Ogihara, T Tanabe, S Baba |
Journal | Circulation
(Circulation)
Vol. 100
Issue 22
Pg. 2231-6
(Nov 30 1999)
ISSN: 1524-4539 [Electronic] United States |
PMID | 10577996
(Publication Type: Journal Article)
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Chemical References |
- Cytochrome P-450 Enzyme System
- Intramolecular Oxidoreductases
- prostacyclin synthetase
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Topics |
- Adult
- Aged
- Asian People
(genetics)
- Base Sequence
- Blood Pressure
(genetics)
- Chromosomes, Human, Pair 20
(genetics)
- Cytochrome P-450 Enzyme System
(genetics)
- DNA Mutational Analysis
- Diastole
- Female
- Gene Frequency
- Genes, Reporter
- Genetic Predisposition to Disease
- Genotype
- Humans
- Hypertension
(epidemiology, genetics, physiopathology)
- Intramolecular Oxidoreductases
(genetics)
- Japan
(epidemiology)
- Male
- Middle Aged
- Minisatellite Repeats
- Molecular Sequence Data
- Multivariate Analysis
- Myocardial Infarction
(genetics)
- Risk Factors
- Systole
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