Previous work has shown, that
stobadine-hydrochloride (-)cis-2,8-dimethyl-2,3,4,4a,5,9b-hexahydro-14-pyrido(4,3b)
indole administered in a single dose 2 mg/kg of
body weight reduces cardiotoxic effect of
isoproterenol (1 mg/kg) as shown by lowered serum
enzyme activities of AST, CPK, LDH and ALT. We studied the effect of
stobadine in vivo on respiration, the level of
ATP,
malondialdehyde (MDA) and superoxiddismutase (SOD) in heart mitochondria. Serum
enzyme activities of AST, CPK and LDH after
stobadine application were significantly decreased. In mitochondria, respiration and activity of SOD were inhibited, level of MDA was increased and level of
ATP was unchanged. The cardioprotective effect of
stobadine is not linked to preservation of mitochondrial function. This effect is probably more complex and mediated on the level of the whole organism.