Effect of multidrug resistance-reversing agents on transporting activity of human canalicular multispecific organic anion transporter.

Abstract

The canalicular multispecific organic anion transporter (cMOAT), also termed MRP2, is a recently identified ATP-binding cassette transporter. We previously established stable human cMOAT cDNA-transfected cells, LLC/cMOAT-1 from LLC-PK1 cells, and LLC/CMV cells that were transfected with an empty vector. We found that LLC/cMOAT-1 cells have increased resistance to vincristine (VCR), 7-ethyl-10-hydroxy-camptothecin, and cisplatin but not to etoposide. The multidrug resistance-reversing agents cyclosporin A (CsA) and 2-[4-(diphenylmethyl)-1-piperazinyl]-5-(trans-4,6-dimethyl-1,3, 2-dioxaphosphorinan-2-yl)-2, 6-dimethyl-4-(3-nitrophenyl)-3-pyridinecarboxylate P-oxide (PAK-104P) almost completely reversed the resistance to VCR, 7-ethyl-10-hydroxy-camptothecin, and cisplatin of LLC/cMOAT-1 cells; and DL-buthionine-(S,R)-sulfoximine, (3'-oxo-4-butenyl-4-methyl-threonine(1), (valine(2)) cyclosporin (PSC833), and 3-([(3-(2-[7-chloro-2-quinolinyl]ethenyl)phenyl)-((3-dimethylamino-3- oxopropyl)-thio)-methyl]thio)propanoic acid (MK571) partially reversed the resistance to these drugs. CsA and PAK-104P at 10 microM enhanced the accumulation of VCR in LLC/cMOAT-1 cells almost to the level in LLC/CMV cells without the agents. The efflux of VCR from LLC/cMOAT-1 cells was enhanced compared with LLC/CMV cells and inhibited by CsA and PAK-104P. Transport of leukotriene C(4) (LTC(4)) and S-(2, 4-dinitrophenyl)glutathione also was studied with membrane vesicles prepared from these cells. LTC(4) and S-(2, 4-dinitrophenyl)glutathione were actively transported into membrane vesicles prepared from LLC/cMOAT-1 cells. The K(m) and V(max) values for the uptake of LTC(4) by the LLC/cMOAT-1 membrane vesicles were 0. 26 +/- 0.05 microM and 7.48 +/- 0.67 pmol/min/mg protein, respectively. LTC(4) transport was competitively inhibited by PAK-104P, CsA, MK571, and PSC833, with K(i) values of 3.7, 4.7, 13.1, and 28.9 microM, respectively. These findings demonstrate that cMOAT confers a novel drug-resistance phenotype. CsA and PAK-104P may be useful for reversing cMOAT-mediated drug resistance in tumors.

• Authors: Z S Chen, T Kawabe, M Ono, S Aoki, T Sumizawa, T Furukawa, T Uchiumi, M Wada, M Kuwano, S I Akiyama
• Journal: Molecular pharmacology (Mol Pharmacol) Vol. 56 Issue 6 Pg. 1219-28 (Dec 1999) ISSN: 0026-895X [Print] United States
• PMID: 10570049 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anion Transport Proteins
• Antineoplastic Agents
• Carrier Proteins
• Cyclic P-Oxides
• Nicotinic Acids
• Propionates
• Quinolines
• Tritium
• PAK 104P
• S-(2,4-dinitrophenyl)glutathione
• Leukotriene C4
• Vincristine
• verlukast
• Cyclosporine
• Adenosine Triphosphate
• Glutathione

Topics

• Adenosine Triphosphate (metabolism)
• Animals
• Anion Transport Proteins
• Antineoplastic Agents (pharmacology)
• Biological Transport (drug effects)
• Carrier Proteins (drug effects, genetics, metabolism)
• Cells, Cultured
• Cyclic P-Oxides (pharmacology)
• Cyclosporine (pharmacology)
• Drug Interactions
• Drug Resistance, Multiple (physiology)
• Glutathione (analogs & derivatives, metabolism)
• Humans
• Kinetics
• Leukotriene C4 (metabolism)
• Nicotinic Acids (pharmacology)
• Osmolar Concentration
• Propionates (pharmacology)
• Quinolines (pharmacology)
• Swine
• Transfection
• Tritium
• Vincristine (pharmacology)