Characterization of a periplasmic ATP-binding cassette iron import system of Brachyspira (Serpulina) hyodysenteriae.

Abstract

The nucleotide sequence of the pathogenic spirochete Brachyspira hyodysenteriae bit (for "Brachyspira iron transport") genomic region has been determined. The bit region is likely to encode an iron ATP-binding cassette transport system with some homology to those encountered in gram-negative bacteria. Six open reading frames oriented in the same direction and physically linked have been identified. This system possesses a protein containing ATP-binding motifs (BitD), two hydrophobic cytoplasmic membrane permeases (BitE and BitF), and at least three lipoproteins (BitA, BitB, and BitC) with homology to iron periplasmic binding proteins. These periplasmic binding proteins exhibit lipoprotein features. They are labeled by [(3)H]palmitate when tested in recombinant Escherichia coli, and their signal peptides are typical for substrates of the type II secretory peptidase. The FURTA system and Congo red assay indicate that BitB and BitC are involved in iron binding. The Bit system is detected only in B. hyodysenteriae and is absent from B. innocens and B. pilosicoli.

• Authors: D Dugourd, C Martin, C R Rioux, M Jacques, J Harel
• Journal: Journal of bacteriology (J Bacteriol) Vol. 181 Issue 22 Pg. 6948-57 (Nov 1999) ISSN: 0021-9193 [Print] United States
• PMID: 10559160 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ATP-Binding Cassette Transporters
• Bacterial Proteins
• Repressor Proteins
• ferric uptake regulating proteins, bacterial
• Iron

Topics

• ATP-Binding Cassette Transporters (chemistry, genetics, metabolism)
• Bacterial Proteins (metabolism)
• Biological Transport
• Blotting, Southern
• Blotting, Western
• Brachyspira hyodysenteriae (genetics, metabolism)
• Cloning, Molecular
• Genomic Library
• Iron (metabolism)
• Molecular Sequence Data
• Periplasm (metabolism)
• Phylogeny
• Precipitin Tests
• Repressor Proteins (metabolism)
• Restriction Mapping
• Sequence Analysis, DNA