Nociceptin (1 - 7) antagonizes nociceptin-induced hyperalgesia in mice.

Abstract

Nociceptin and its N-terminal fragment, nociceptin (1 7), were administered intrathecally (i.t.) into conscious mice. Nociceptin (3.0 fmol) produced a significant reduction in the nociceptive thermal threshold (hyperalgesia) measured as the tail-flick and paw-withdrawal responses. Nociceptin (1-7), injected i.t., at 150-1200 fmol had no significant effect. However, when nociceptin (1-7) (150 1200 fmol) was injected simultaneously with nociceptin (3.0 fmol), nociceptin-induced hyperalgesia was significantly reduced. Analgesia induced by a high dose (1200 pmol) of nociceptin was not antagonized by co-administration of nociceptin (1-7) (1200 fmol). These results suggest that N-terminal fragments of nociceptin formed endogenously could modulate the hyperalgesic action of nociceptin in the spinal cord.

• Authors: T Sakurada, S Sakurada, S Katsuyama, C Sakurada, K Tan-No, L Terenius
• Journal: British journal of pharmacology (Br J Pharmacol) Vol. 128 Issue 5 Pg. 941-4 (Nov 1999) ISSN: 0007-1188 [Print] England
• PMID: 10556929 (Publication Type: Journal Article)

Chemical References

• Opioid Peptides
• Peptide Fragments
• Receptors, Opioid
• nociceptin
• Nociceptin Receptor
• Oprl1 protein, mouse

Topics

• Amino Acid Sequence
• Animals
• Hyperalgesia (chemically induced, prevention & control)
• Injections, Intraventricular
• Injections, Spinal
• Male
• Mice
• Molecular Sequence Data
• Opioid Peptides (antagonists & inhibitors)
• Pain Measurement (drug effects)
• Peptide Fragments (administration & dosage, chemical synthesis, pharmacology)
• Reaction Time (drug effects)
• Receptors, Opioid (metabolism)
• Spinal Cord (drug effects, metabolism)
• Nociceptin Receptor