| Abstract | Psoralen in conjunction with UVA (PUVA) is perhaps the most effective treatment for psoriasis. It is, however, a risk factor for skin cancer in these patients and there is a need to develop non-invasive assays reflective of treatment-induced DNA damage. We report here the assessment of two important lesions, thymine dimer (T<>T) and 8-oxo-2'-deoxyguanosine (8-OHdG), in the urine of psoriasis patients. It was found that, once corrected for urine concentration, the psoriatic group had significantly higher (P<0. 0001) urinary levels of thymine dimers compared to the control group. No significant differences in urinary 8-OHdG levels were noted between the psoriatic, atopic dermatitis and control groups. Therefore biomonitoring of therapy from the very start with this simple and non-invasive assay could perhaps be an effective measure of the risk involved with the treatment allowing optimization for minimal-risk therapy. |
| Authors | J Ahmad, M S Cooke, A Hussieni, M D Evans, K Patel, R M Burd, T O Bleiker, P E Hutchinson, J Lunec
(Affiliation: Division of Chemical Pathology, Centre for Mechanisms of Human Toxicity, PO Box 138, University of Leicester, Lancaster Road, Leicester, UK.)
|
| Journal | FEBS letters
(FEBS Lett)
Vol. 460
Issue 3
Pg. 549-53
(Nov 5 1999)
ISSN: 0014-5793 NETHERLANDS |
| PMID | 10556533
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Pyrimidine Dimers
- Poly T
- 8-oxo-7-hydrodeoxyguanosine
- Deoxyguanosine
|
| Topics |
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- DNA Damage
(genetics)
- Deoxyguanosine
(analogs & derivatives, urine)
- Female
- Humans
- Male
- Middle Aged
- PUVA Therapy
(adverse effects)
- Poly T
(therapeutic use)
- Psoriasis
(drug therapy, urine)
- Pyrimidine Dimers
(urine)
- Risk Assessment
|
