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The PTEN lipid phosphatase domain is not required to inhibit invasion of glioma cells.

Abstract
The tumor suppressor PTEN negatively controls the phosphoinositide 3-kinase pathway for cell survival by dephosphorylating the phospholipid substrates phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. PTEN has been proposed to dephosphorylate focal adhesion kinase and is implicated in the regulation of cell spreading and motility. We analyzed the role of PTEN in invasion using the two highly infiltrative glioma cell lines U87MG (which lacks functional PTEN) and LN229 (wild-type PTEN). After constitutive overexpression of wild-type and phosphatase-deficient (C124S) PTEN, we found significant inhibition of invasion (50-70%) independent of the PTEN status of the cell and of the catalytic core domain of PTEN. Although wild-type but not mutant (C124S) PTEN decreased PKB/Akt phosphorylation and induced a stellate morphology in U87MG cells, an accompanying reduction of focal adhesion kinase phosphorylation was not seen. We conclude that phosphatase-independent domains of PTEN markedly reduced the invasive potential of glioma cells, defining a structural role for PTEN that regulates cell motility distinct of the PKB/Akt pathway.
AuthorsD Maier, G Jones, X Li, A H Schönthal, O Gratzl, E G Van Meir, A Merlo
JournalCancer research (Cancer Res) Vol. 59 Issue 21 Pg. 5479-82 (Nov 01 1999) ISSN: 0008-5472 [Print] United States
PMID10554022 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Cell Adhesion Molecules
  • DNA, Complementary
  • Tumor Suppressor Proteins
  • Protein-Tyrosine Kinases
  • Focal Adhesion Kinase 1
  • Focal Adhesion Protein-Tyrosine Kinases
  • PTK2 protein, human
  • Phosphoric Monoester Hydrolases
  • PTEN Phosphohydrolase
  • PTEN protein, human
Topics
  • Brain Neoplasms (metabolism)
  • Cell Adhesion Molecules (metabolism)
  • Cell Movement
  • DNA, Complementary (metabolism)
  • Focal Adhesion Kinase 1
  • Focal Adhesion Protein-Tyrosine Kinases
  • Gene Expression Regulation, Neoplastic
  • Glioma (metabolism)
  • Humans
  • Immunohistochemistry
  • Mutation
  • Neoplasm Invasiveness
  • PTEN Phosphohydrolase
  • Phenotype
  • Phosphoric Monoester Hydrolases (metabolism)
  • Phosphorylation
  • Protein-Tyrosine Kinases (metabolism)
  • Signal Transduction
  • Transfection
  • Tumor Cells, Cultured
  • Tumor Suppressor Proteins

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