Characterization by gas chromatography/mass spectrometry of sterols in saccharomyces cerevisiae during autolysis.

Abstract

Yeast autolysis affects membrane stability and induces a release of vacuolar enzymes into the cell cytoplasm. Consecutively, it was important to study the evolution of sterol content in Saccharomycescerevisiae for a fourteen day period of accelerated autolysis. Unesterified and esterified sterols were analyzed both in the biomass and in the autolysis medium. Ten sterols were identified by gas chromatography/mass spectrometry. A second group of six sterols was separated and partially characterized. Among the first group of 10 sterols, a dehydroergosterol was identified as ergosta-5, 7,9(11),22-tetraen-3beta-ol, not yet charaterized in S. cerevisiae. Yeast autolysis induced a decrease of esterified sterol content, especially first intermediates in the sequence of the ergosterol biosynthesis, as zymosterol. In contrast, the yeast autolysis resulted in the release of a low quantity of sterols into the medium. At the end of the fourteenth day of autolysis, 0.015% of the total sterol content of the initial biomass was found in the medium.

• Authors: Y Le Fur, G Maume, M Feuillat, B F Maume
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 47 Issue 7 Pg. 2860-4 (Jul 1999) ISSN: 0021-8561 [Print] United States
• PMID: 10552576 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Sterols

Topics

• Autolysis
• Gas Chromatography-Mass Spectrometry
• Saccharomyces cerevisiae (chemistry)
• Sterols (chemistry)