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A biochemical genomics approach for identifying genes by the activity of their products.

Abstract
For the identification of yeast genes specifying biochemical activities, a genomic strategy that is rapid, sensitive, and widely applicable was developed with an array of 6144 individual yeast strains, each containing a different yeast open reading frame (ORF) fused to glutathione S-transferase (GST). For the identification of ORF-associated activities, strains were grown in defined pools, and GST-ORFs were purified. Then, pools were assayed for activities, and active pools were deconvoluted to identify the source strains. Three previously unknown ORF-associated activities were identified with this strategy: a cyclic phosphodiesterase that acts on adenosine diphosphate-ribose 1"-2" cyclic phosphate (Appr>p), an Appr-1"-p-processing activity, and a cytochrome c methyltransferase.
AuthorsM R Martzen, S M McCraith, S L Spinelli, F M Torres, S Fields, E J Grayhack, E M Phizicky
JournalScience (New York, N.Y.) (Science) Vol. 286 Issue 5442 Pg. 1153-5 (Nov 05 1999) ISSN: 0036-8075 [Print] United States
PMID10550052 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Fungal Proteins
  • Recombinant Fusion Proteins
  • adenosine diphosphate-ribose 1''-2''cyclic phosphate
  • Adenosine Diphosphate Ribose
  • cytochrome C methyltransferase
  • Histone-Lysine N-Methyltransferase
  • Glutathione Transferase
  • Phosphoric Diester Hydrolases
Topics
  • Adenosine Diphosphate Ribose (analogs & derivatives, metabolism)
  • Fungal Proteins (genetics, metabolism)
  • Genes, Fungal
  • Genetic Techniques
  • Glutathione Transferase (genetics)
  • Histone-Lysine N-Methyltransferase (genetics, isolation & purification, metabolism)
  • Open Reading Frames
  • Phosphoric Diester Hydrolases (genetics, isolation & purification, metabolism)
  • Recombinant Fusion Proteins (isolation & purification)
  • Saccharomyces cerevisiae (genetics)
  • Sensitivity and Specificity

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