Abstract |
For the identification of yeast genes specifying biochemical activities, a genomic strategy that is rapid, sensitive, and widely applicable was developed with an array of 6144 individual yeast strains, each containing a different yeast open reading frame (ORF) fused to glutathione S-transferase (GST). For the identification of ORF-associated activities, strains were grown in defined pools, and GST-ORFs were purified. Then, pools were assayed for activities, and active pools were deconvoluted to identify the source strains. Three previously unknown ORF-associated activities were identified with this strategy: a cyclic phosphodiesterase that acts on adenosine diphosphate-ribose 1"-2" cyclic phosphate (Appr>p), an Appr-1"-p-processing activity, and a cytochrome c methyltransferase.
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Authors | M R Martzen, S M McCraith, S L Spinelli, F M Torres, S Fields, E J Grayhack, E M Phizicky |
Journal | Science (New York, N.Y.)
(Science)
Vol. 286
Issue 5442
Pg. 1153-5
(Nov 05 1999)
ISSN: 0036-8075 [Print] United States |
PMID | 10550052
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Fungal Proteins
- Recombinant Fusion Proteins
- adenosine diphosphate-ribose 1''-2''cyclic phosphate
- Adenosine Diphosphate Ribose
- cytochrome C methyltransferase
- Histone-Lysine N-Methyltransferase
- Glutathione Transferase
- Phosphoric Diester Hydrolases
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Topics |
- Adenosine Diphosphate Ribose
(analogs & derivatives, metabolism)
- Fungal Proteins
(genetics, metabolism)
- Genes, Fungal
- Genetic Techniques
- Glutathione Transferase
(genetics)
- Histone-Lysine N-Methyltransferase
(genetics, isolation & purification, metabolism)
- Open Reading Frames
- Phosphoric Diester Hydrolases
(genetics, isolation & purification, metabolism)
- Recombinant Fusion Proteins
(isolation & purification)
- Saccharomyces cerevisiae
(genetics)
- Sensitivity and Specificity
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