Abstract | OBJECTIVE: To define the pattern of mRNA expression of all human matrix metalloproteinases ( MMPs) described to date in rheumatoid arthritis (RA) and traumatic synovial membrane, in order to differentiate between a physiological tissue remodelling pattern and that associated with inflammatory tissue destruction. METHODS: RESULTS: MMP-2 ( gelatinase A), MMP-3 (stromelysin-1), MMP-11 (stromelysin-3) and MMP-19 were constitutively expressed. MMP-1 (fibroblast type collagenase), MMP-9 ( gelatinase B) and MMP-14 (MT1-MMP) were expressed in all RA, but only in 55-80% of trauma samples. MMP-13 (collagenase-3) and MMP-15 (MT2-MMP) were expressed exclusively in RA (80-90% of the samples). MMP-20 ( enamelysin) was absent and MMP-8 (collagenase-2) was rarely found in RA or trauma. All other MMPs (-7, -10, -12, -16, -17) had an intermediate pattern of expression. CONCLUSIONS: Some MMPs without interstitial collagenase activity seem to have a constitutive pattern of expression and probably participate in physiological synovial tissue remodelling. Some MMPs are exclusively associated to RA synovitis, for example, MMP-13, which preferentially degrades type II collagen and aggrecan, and MMP-15, which activates proMMP-2 and proMMP-13 and is involved in tumour necrosis factor alpha processing. This clear cut rheumatoid/inflammatory MMP profile, more complex than has been previously appreciated, may facilitate inflammatory tissue destruction in RA.
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Authors | Y T Konttinen, M Ainola, H Valleala, J Ma, H Ida, J Mandelin, R W Kinne, S Santavirta, T Sorsa, C López-Otín, M Takagi |
Journal | Annals of the rheumatic diseases
(Ann Rheum Dis)
Vol. 58
Issue 11
Pg. 691-7
(Nov 1999)
ISSN: 0003-4967 [Print] England |
PMID | 10531073
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- RNA, Messenger
- Matrix Metalloproteinases
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Arthritis, Rheumatoid
(enzymology)
- Female
- Gene Expression
- Humans
- Male
- Matrix Metalloproteinases
(genetics, metabolism)
- Middle Aged
- RNA, Messenger
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Synovial Membrane
(enzymology, injuries)
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