Abstract |
The human nm23-H1 gene product, a putative metastasis suppressor, was identified as nucleoside diphosphate kinase (NDPK) A isoform. To investigate the functional effect of nm23-H1's NDPK activity on its suppression of the components of metastatic phenotype, we transfected a human prostate carcinoma cell line, DU145, with the cDNA encoding nm23-H1 mutant protein lacking NDPK activity. The mutant nm23-H1 transfected cell lines displayed decreased invasiveness and colonization in soft agar as the wild-type nm23-H1 transfectants did when compared with the control transfected line. The results suggest that the metastasis suppressing function of nm23-H1 is independent of the NDPK enzymatic activity.
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Authors | H Y Lee, H Lee |
Journal | Cancer letters
(Cancer Lett)
Vol. 145
Issue 1-2
Pg. 93-9
(Oct 18 1999)
ISSN: 0304-3835 [Print] Ireland |
PMID | 10530775
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- NM23 Nucleoside Diphosphate Kinases
- Transcription Factors
- NME1 protein, human
- Nucleoside-Diphosphate Kinase
- Monomeric GTP-Binding Proteins
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Topics |
- Carcinoma
(drug therapy, pathology, secondary)
- Humans
- Male
- Monomeric GTP-Binding Proteins
- Mutation
- NM23 Nucleoside Diphosphate Kinases
- Neoplasm Invasiveness
- Nucleoside-Diphosphate Kinase
(metabolism)
- Phenotype
- Prostatic Neoplasms
(drug therapy, enzymology, pathology)
- Transcription Factors
(genetics, therapeutic use)
- Transfection
- Tumor Cells, Cultured
- Tumor Stem Cell Assay
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