Identification and molecular characterization of BP75, a novel bromodomain-containing protein.

Abstract	We here describe the identification and characterization of a novel bromodomain-containing protein, the bromodomain protein of 75 kDa (BP75). Initially, we identified BP75 in a two-hybrid screening for proteins that interact with the first PDZ (acronym for post-synaptic density protein PSD-95, Drosophila discs large tumor suppressor DlgA and the tight junction protein ZO-1) domain in protein tyrosine phosphatase-BAS-like (PTP-BL). We found that BP75 is expressed ubiquitously and show that both BP75 and a PTP-BL deletion mutant consisting of the first PDZ domain are located mainly in the nucleus, although cytoplasmic localization is also evident. Full-length PTP-BL, on the contrary, is predominantly localized in the cytoplasm, although some basal nuclear staining is observed. The described molecular interaction may reflect a mechanism of coupling submembraneous signalling events and nuclear events.
Authors	E Cuppen, M van Ham, B Pepers, B Wieringa, W Hendriks
Journal	FEBS letters (FEBS Lett) Vol. 459 Issue 3 Pg. 291-8 (Oct 15 1999) ISSN: 0014-5793 [Print] England
PMID	10526152 (Publication Type: Journal Article)
Chemical References	Brd7 protein, mouse Carrier Proteins Chromosomal Proteins, Non-Histone DNA, Complementary Disks Large Homolog 4 Protein Dlg4 protein, mouse Intracellular Signaling Peptides and Proteins Membrane Proteins Nerve Tissue Proteins Nuclear Proteins postsynaptic density proteins Guanylate Kinases Protein Tyrosine Phosphatase, Non-Receptor Type 13 Protein Tyrosine Phosphatases Ptpn13 protein, mouse Bromine
Topics	3T3 Cells Amino Acid Sequence Animals Bromine (chemistry) COS Cells Carrier Proteins (genetics, isolation & purification, metabolism) Chromosomal Proteins, Non-Histone Conserved Sequence DNA, Complementary (metabolism) Disks Large Homolog 4 Protein Guanylate Kinases Intracellular Signaling Peptides and Proteins Membrane Proteins Mice Mice, Inbred C57BL Molecular Sequence Data Nerve Tissue Proteins (metabolism) Nuclear Proteins (genetics, isolation & purification, metabolism) Protein Structure, Tertiary Protein Tyrosine Phosphatase, Non-Receptor Type 13 Protein Tyrosine Phosphatases (chemistry, metabolism) Sequence Homology, Amino Acid Subcellular Fractions (metabolism) Two-Hybrid System Techniques

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