Abstract | BACKGROUND: It is suggested that urinary eosinophil protein X (EPX) is a noninvasive tool to monitor bronchial inflammation in asthmatic children. However, circadian variation of the number and activation of eosinophils might possibly influence urinary EPX excretion. OBJECTIVE: METHODS: Urinary EPX excretion (microg/mmol creatinine) was measured in the morning and afternoon in 22 stable asthmatics and in 16 nonatopic, nonasthmatic controls to investigate circadian variation. Additionally, EPX excretion in the afternoon was analysed in 21 children with chronic asthma before and after 6 weeks of treatment with inhaled corticosteroids, and in seven children within 24 h of admission due to an asthma exacerbation and again 3 months after discharge. RESULTS: EPX excretion in the first morning urine sample of the day compared with the afternoon urine sample was significantly higher both in children with asthma (n = 22; mean +/- standard deviation: 179.7 +/- 97.3 vs 60.9 +/- 40.7 microg/mmol creatinine, P = 0.0001) and in nonatopic nonasthmatic controls (n = 16; 114.5 +/- 57.1 vs 53.4 +/- 29.0 microg/mmol creatinine, P = 0.0001). EPX excretion decreased significantly after 6 weeks of anti-inflammatory treatment in the group of children with chronic asthma (n = 21; 124.7 +/- 84.6 vs 87. 5 +/- 61.9 microg/mmol creatinine, P = 0.02) and in the group of children with an acute asthma exacerbation 3 months after discharge (n = 7; 233.2 +/- 174.5 vs 75.8 +/- 59.5 microg/mmol creatinine, P = 0.02). CONCLUSION: This study suggests a circadian variation of EPX excretion in children with asthma and in nonatopic, nonasthmatic controls. Measurement of EPX excretion is helpful monitoring therapy in asthmatic children if circadian variation is considered.
|
Authors | K Storm van's Gravesande, J Mattes, T Grüntjens, M Kopp, H H Seydewitz, M Moseler, J Kuehr |
Journal | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
(Clin Exp Allergy)
Vol. 29
Issue 11
Pg. 1497-501
(Nov 1999)
ISSN: 0954-7894 [Print] England |
PMID | 10520077
(Publication Type: Journal Article)
|
Chemical References |
- Anti-Inflammatory Agents
- Blood Proteins
- Budesonide
- Eosinophil-Derived Neurotoxin
- Ribonucleases
|
Topics |
- Adolescent
- Anti-Inflammatory Agents
(therapeutic use)
- Asthma
(drug therapy, immunology, urine)
- Blood Proteins
(urine)
- Budesonide
(therapeutic use)
- Child
- Child, Preschool
- Circadian Rhythm
- Eosinophil-Derived Neurotoxin
- Eosinophils
(immunology)
- Female
- Humans
- Male
- Ribonucleases
|