The cytoplasmic domain of C-CAM1 tumor suppressor is necessary and sufficient for suppressing the tumorigenicity of prostate cancer cells.

Abstract	We have previously shown that C-CAM1 cell adhesion molecule can suppress the growth of prostate cancer cells in vivo. In this study, we determined the minimal domain of C-CAM1 that is required for its tumor-suppressive activity. DU145 prostate cancer cells were infected with recombinant adenoviruses containing various C-CAM1 mutant genes, and the effects of the mutant C-CAM1 proteins on the growth of DU145 cells were assessed in a nude-mice xenograft model. Deletion of C-CAM1's cytoplasmic domain, which is not required for its adhesion activity, abolished the growth-suppressive activity, whereas deletion of the adhesion domain did not. This observation suggests that C-CAM1's extracellular domain may be not essential for its tumor suppressive activity. Indeed, we found that expression of the C-CAM1 cytoplasmic domain alone led to growth suppression of DU145 cells. These results suggest that the cytoplasmic domain of C-CAM1 is necessary and sufficient for its growth-suppressive function.
Authors	V T Estrera, W Luo, D Phan, K Earley, D C Hixson, S H Lin
Journal	Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 263 Issue 3 Pg. 797-803 (Oct 05 1999) ISSN: 0006-291X [Print] United States
PMID	10512760 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Copyright	Copyright 1999 Academic Press.
Chemical References	Antigens, CD CD66 antigens Carcinoembryonic Antigen Ceacam1 protein, mouse Ceacam2 protein, mouse Cell Adhesion Molecules Glycoproteins Recombinant Proteins Adenosine Triphosphatases
Topics	Adenosine Triphosphatases (chemistry, genetics, physiology) Adenoviridae Animals Antigens, CD Carcinoembryonic Antigen Cell Adhesion Cell Adhesion Molecules (chemistry, genetics, physiology) Cell Aggregation Cell Division Cloning, Molecular Cytoplasm (metabolism) Genetic Vectors Glycoproteins Humans Male Mice Mice, Nude Mutagenesis, Site-Directed Prostatic Neoplasms (pathology, physiopathology) Recombinant Proteins (chemistry, metabolism) Transfection

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