Abstract | OBJECTIVE:
Fluorescent dyes are commonly used as probes for assessment of macromolecular permeability. Despite numerous examples of light- dye induced toxicity in the microvasculature, little is known regarding the relative phototoxicity of commonly used fluorescent conjugates. We, therefore, compared the phototoxicity of four fluorescent conjugates of bovine serum albumin (BSA) available commercially. METHODS: An in vitro photohemolysis assay was used, in which rat erythrocytes were incubated with fluorescently labeled BSA and exposed to epi-illumination, using an inverted microscope designed for microvascular permeability experiments. Photohemolysis was quantified by monitoring light transmission across the cells. RESULTS: CONCLUSIONS: Relative phototoxicity of fluorescent albumin conjugates differs considerably. Selection of fluorescent conjugates for use in microvascular experiments based on phototoxicity should consider both the type and molar content of the fluorochrome.
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Authors | R E Rumbaut, A J Sial |
Journal | Microcirculation (New York, N.Y. : 1994)
(Microcirculation)
Vol. 6
Issue 3
Pg. 205-13
(Sep 1999)
ISSN: 1073-9688 [Print] United States |
PMID | 10501094
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Fluorescent Dyes
- Serum Albumin, Bovine
- Histidine
- Fluorescein-5-isothiocyanate
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Topics |
- Animals
- Capillary Permeability
- Dose-Response Relationship, Drug
- Erythrocytes
(drug effects, radiation effects)
- Fluorescein-5-isothiocyanate
(toxicity)
- Fluorescent Dyes
(toxicity)
- Hemolysis
(drug effects, radiation effects)
- Histidine
(pharmacology)
- Light
(adverse effects)
- Male
- Photochemistry
- Rats
- Rats, Sprague-Dawley
(blood)
- Serum Albumin, Bovine
(chemistry)
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