Parathyroid hormone-related protein is an independent prognostic factor for renal cell carcinoma.

Abstract

BACKGROUND:

Parathyroid hormone-related protein (PTHrP) has been shown to be the principal cause of humoral hypercalcemia associated with renal cell carcinoma (RCC). Recent studies have demonstrated that the amino-terminal region of PTHrP has growth factor-like activities, suggesting it may play a role in the development of RCC. In this study, expression of the carboxy-terminal region of PTHrP was assessed immunohistochemically and its significance in predicting the prognosis of RCC was studied.

METHODS:

Forty radical nephrectomy specimens were immunostained with a murine monoclonal antibody (9H7) against the carboxy-terminal region (amino acids 109-141) of PTHrP using the streptavidin-peroxidase enzyme conjugate method. Staining intensity was evaluated semiquantitatively and compared with clinicopathologic features of the corresponding RCC.

RESULTS:

Immunoreactivity to 9H7 was observed to be localized to the cytosol of tumor cells at various staining intensities. There were 30 cases (75.0%) with strong staining and 10 cases (25.0%) in which staining was weak or nonexistent. Staining intensity showed no significant correlation with gender, tumor greatest dimension, stage, or grade. Tumors of the clear cell type expressed PTHrP to a significantly greater extent than tumors of the granular cell type. Tumor recurrence was significantly greater in the weakly stained or unstained group compared with the strongly stained group (P = 0.035). Multivariate analysis indicated that PTHrP expression and tumor stage were equally significant prognostic indicators in RCCs measuring <10 cm in greatest dimension.

CONCLUSIONS:

Evident PTHrP(109-141) expression is present in the majority of RCCs. The results of the current study indicate PTHrP(109-141) may be a possible marker of cellular differentiation and may be useful for predicting recurrence free survival in RCC patients after radical nephrectomy.

• Authors: M Iwamura, W Wu, M Muramoto, M Ohori, S Egawa, T Uchida, S Baba
• Journal: Cancer (Cancer) Vol. 86 Issue 6 Pg. 1028-34 (Sep 15 1999) ISSN: 0008-543X [Print] United States
• PMID: 10491530 (Publication Type: Journal Article)
• Copyright: Copyright 1999 American Cancer Society.

Chemical References

• Antibodies
• Neoplasm Proteins
• PTHLH protein, human
• Parathyroid Hormone-Related Protein
• Peptide Fragments
• Proteins
• parathyroid hormone-related protein (109-141)

Topics

• Adult
• Aged
• Aged, 80 and over
• Antibodies
• Carcinoma, Renal Cell (chemistry, pathology, surgery)
• Cytosol (chemistry)
• Disease-Free Survival
• Female
• Humans
• Immunohistochemistry
• Kidney Neoplasms (chemistry, pathology, surgery)
• Kidney Tubules (chemistry)
• Lymph Node Excision
• Male
• Middle Aged
• Multivariate Analysis
• Neoplasm Proteins (analysis)
• Neoplasm Recurrence, Local (pathology)
• Nephrectomy
• Parathyroid Hormone-Related Protein
• Peptide Fragments (immunology)
• Prognosis
• Proteins (analysis, immunology)