EGb 761 protects liver mitochondria against injury induced by in vitro anoxia/reoxygenation.

Abstract	The present study investigated the protective effects of Ginkgo biloba extract (EGb 761) on rat liver mitochondrial damage induced by in vitro anoxia/reoxygenation. Anoxia/reoxygenation was known to impair respiratory activities and mitochondrial oxidative phosphorylation efficiency. ADP/O (2.57 +/- 0.11) decreased after anoxia/reoxygenation (1.75 +/- 0.09, p < .01), as well as state 3 and uncoupled respiration (-20%, p < .01), but state 4 respiration increased (p < .01). EGb 761 (50-200 microg/ml) had no effect on mitochondrial functions before anoxia, but had a specific dose-dependent protective effect after anoxia/reoxygenation. When mitochondria were incubated with 200 microg/ml EGb 761, they showed an increase in ADP/O (2.09 +/- 0.14, p < .05) and a decrease in state 4 respiration (-22%) after anoxia/reoxygenation. In EPR spin-trapping measurement, EGb 761 decreased the EPR signal of superoxide anion produced during reoxygenation. In conclusion, EGb 761 specially protects mitochondrial ATP synthesis against anoxia/reoxygenation injury by scavenging the superoxide anion generated by mitochondria.
Authors	G Du, K Willet, A Mouithys-Mickalad, C M Sluse-Goffart, M T Droy-Lefaix, F E Sluse
Journal	Free radical biology & medicine (Free Radic Biol Med) Vol. 27 Issue 5-6 Pg. 596-604 (Sep 1999) ISSN: 0891-5849 [Print] United States
PMID	10490280 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Flavonoids Free Radical Scavengers Plant Extracts Spin Labels Superoxides Ginkgo biloba extract Adenosine Triphosphate Xanthine Oxidase Oxygen
Topics	Adenosine Triphosphate (biosynthesis) Animals Electron Spin Resonance Spectroscopy Flavonoids (pharmacology) Free Radical Scavengers (pharmacology) Ginkgo biloba (therapeutic use) Hypoxia (pathology) Male Mitochondria, Liver (drug effects, pathology) Oxidative Phosphorylation (drug effects) Oxygen (pharmacology) Oxygen Consumption (drug effects) Phytotherapy Plant Extracts Plants, Medicinal Rats Rats, Wistar Spin Labels Superoxides (pharmacology) Xanthine Oxidase

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