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Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion.

Abstract
The incidence of false positives due to the presence of bacterial DNA in Taq DNA polymerase is an obstacle to the use of PCR in the diagnosis of infection. We describe a method that uses a restriction enzyme to destroy the ability of contaminating sequences to act as templates for a nested PCR which uses primers based on the 16S rRNA genes. The method was used prior to a PCR that amplified 10 fg of bacterial DNA. This method can be readily adapted to suit other sensitive PCRs required for clinical applications.
AuthorsN M Carroll, P Adamson, N Okhravi
JournalJournal of clinical microbiology (J Clin Microbiol) Vol. 37 Issue 10 Pg. 3402-4 (Oct 1999) ISSN: 0095-1137 [Print] United States
PMID10488219 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA, Bacterial
  • Taq Polymerase
Topics
  • DNA, Bacterial (isolation & purification)
  • Polymerase Chain Reaction
  • Taq Polymerase (analysis)

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