Abstract |
This study examined the role of Cu as a mediator of cardiac postischemic oxidative injury. Isolated rat hearts were subjected to 20 min of normothermic global ischemia, followed by 30 min of reperfusion; after 20 min of preischemic loading with Krebs-Henseleit buffer +/- 20 or 30 microM zinc-bis-histidinate (Zn-His2), 0.5 mM deferoxamine ( DEF) or 42 microM neocuproine (NEO). Postischemic developed systolic pressure and rate-pressure product were highest and postischemic end-diastolic pressure was lowest in hearts treated with 20 or 30 microM Zn-His2 and 0.5 mM DEF. Cu efflux was significantly increased by 225 and 290% (end of preischemic loading), and 325 and 375% (immediate postischemic period) of control basal rates in hearts treated with 30 microM Zn-His2 and 0.5 mM DEF, respectively. NEO did not effect any of these parameters. By the end of ischemia, protein carbonyls were lowest in Zn-His2-treated hearts and highest in DEF-treated hearts when compared with control hearts. The results of this study suggest that removal of redox-active Cu before ischemia has beneficial effects, indicating a mediatory role in postischemic cardiac oxidative injury.
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Authors | S R Powell, E M Gurzenda, M A Wingertzahn, R A Wapnir |
Journal | The American journal of physiology
(Am J Physiol)
Vol. 277
Issue 3
Pg. H956-62
(09 1999)
ISSN: 0002-9513 [Print] United States |
PMID | 10484416
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antidotes
- Chelating Agents
- Organometallic Compounds
- Phenanthrolines
- zinc bis(histidinate)
- Histidine
- Copper
- neocuproine
- Deferoxamine
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Topics |
- Animals
- Antidotes
(pharmacology)
- Chelating Agents
(pharmacology)
- Copper
(metabolism)
- Deferoxamine
(pharmacology)
- Histidine
(analogs & derivatives, pharmacology)
- Male
- Myocardial Ischemia
(metabolism, physiopathology)
- Myocardial Reperfusion Injury
(metabolism)
- Organometallic Compounds
(pharmacology)
- Oxidative Stress
- Phenanthrolines
(pharmacology)
- Rats
- Rats, Sprague-Dawley
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