5-(N-Ethyl-N-isopropyl)-amiloride, an Na(+)-H(+) exchange inhibitor, protects gerbil hippocampal neurons from ischemic injury.

Abstract

The effect of the selective Na(+)/H(+) antiporter inhibitor 5-(N-ethyl-N-isopropyl)-amiloride (EIPA) on cerebral ischemia/reperfusion injury was evaluated in the Mongolian gerbil. Ischemia was induced in unanaesthetized gerbils by a 5-min period of bilateral common carotid artery occlusion followed by reperfusion for 6 days. Two groups of gerbils were injected intraperitoneally with either dimethyl sulfoxide (DMSO; 10 microl) or EIPA (5 mg/kg in 10 microl DMSO) 30 min prior to ischemia. The increase in locomotor activity in the EIPA-treated group was significantly less than that of the control group at both 24 h and 6-day post-ischemia. The extent of CA1 pyramidal neuron loss was significantly reduced in the EIPA-treated group in comparison with that of DMSO treated controls. These results suggest that EIPA can protect cerebral neurons from ischemia/reperfusion injury and implicates acidosis and Na(+)/H(+) exchange as a causative factor in such injury.

• Authors: J W Phillis, A Y Estevez, L L Guyot, M H O'Regan
• Journal: Brain research (Brain Res) Vol. 839 Issue 1 Pg. 199-202 (Aug 21 1999) ISSN: 0006-8993 [Print] Netherlands
• PMID: 10482815 (Publication Type: Journal Article)

Chemical References

• Neuroprotective Agents
• Sodium-Hydrogen Exchangers
• Amiloride
• ethylisopropylamiloride

Topics

• Amiloride (analogs & derivatives, pharmacology)
• Animals
• Brain Ischemia (prevention & control)
• Gerbillinae
• Hippocampus (blood supply, drug effects, pathology)
• Male
• Neurons (drug effects)
• Neuroprotective Agents (pharmacology)
• Reperfusion Injury (prevention & control)
• Sodium-Hydrogen Exchangers (antagonists & inhibitors)