Modulatory potential of clocimum oil on mouse skin papillomagenesis and the xenobiotic detoxication system.

Abstract

The present study was designed to elucidate the mechanistic inhibitory efficacy of clocimum (an eugenol rich variety of Ocimum gratissimum; Labiatae) oil on murine skin papillomagensis. Topical application of clocimum oil (50 microl/animal/day) during peri-initiation stage (1 week before and 2 weeks after initiation) of 7,12-dimethylbenz[a]anthracene (DMBA)-induced papillomagenesis and/or during the tumour promotion stage reduced (P < 0.05) the (i) tumour burden to 5.00, 4.41 and 4.50 (positive control value 5.27); (ii) cumulative number of papillomas to 85, 75 and 72 (positive control value : 95); and (iii) percent incidence of mice bearing papillomas to 94, 89 and 88, respectively (positive control value 94). Significant (P < 0.01) elevation in the hepatic levels of glutathione S-transferase (GST), sulfhydryl (-SH) and cytochrome b5 (Cyt. b5) was observed by the respective topical treatment of clocimum oil. Even in the skin tissue of the mouse, the topical treatment of clocimum oil enhanced (P < 0.05) the -SH content. The results suggest the modest chemopreventive potential of clocimum oil against the murine skin papillomagenesis, and such effects may be partly due to the modulated xenobiotic detoxication system enzymes.

• Authors: A Singh, S P Singh, R Bamezai
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 37 Issue 6 Pg. 663-70 (Jun 1999) ISSN: 0278-6915 [Print] England
• PMID: 10478835 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Plant Oils
• Xenobiotics
• 9,10-Dimethyl-1,2-benzanthracene
• basil oil

Topics

• 9,10-Dimethyl-1,2-benzanthracene
• Animals
• Chemoprevention
• Disease Progression
• Inactivation, Metabolic
• Male
• Mice
• Ocimum
• Ocimum basilicum (chemistry)
• Papilloma (chemically induced, prevention & control)
• Plant Oils (therapeutic use)
• Skin Neoplasms (chemically induced, prevention & control)
• Xenobiotics (metabolism)