Abstract | PURPOSE:
Interleukin (IL)-1alpha and IL-6 levels in the cornea are greatly elevated during the early stages after an alkali burn in mice. The authors investigated the effect of synthetic inhibitor of matrix metalloproteinase ( SIMP) on the expression of inflammatory cytokines in alkali-burned murine corneas and evaluated the clinical appearance of the eyes. METHODS: After 0.5N NaOH- alkali burns to 400 corneas of ICR mice, 200 received 400 microg/ml of SIMP topically 4 times a day while 200 corneas were similarly treated with vehicle only. At days 4, 7 and 14 after injury, each cornea was assigned a clinical score for corneal opacity, corneal epithelial defect, hyphema and cataract. Extracts of injured corneas in each group were then assayed for cytokine production using ELISA systems for IL-1alpha, IL-1beta, IL-6 and tumor necrosis factor-alpha ( TNF-alpha). RESULTS: The levels of IL-1alpha, IL-1beta and IL-6 were significantly lower in the SIMP-treated group than in the vehicle-treated group 7 days after the burn. However, levels of these cytokines were similar in the SIMP and non- SIMP groups at days 4 and 14. Levels of TNF-alpha did not differ between both groups at any postinjury time. In the SIMP-treated corneas, there was less opacification and hyphema formation and epithelial regeneration was faster. CONCLUSIONS: Topical application of SIMP in alkali-burned murine corneas reduced the expression of IL-1alpha, IL-1beta, and IL-6 and lessened the severity of the injury.
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Authors | C Sotozono, J He, M Tei, Y Honma, S Kinoshita |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 40
Issue 10
Pg. 2430-4
(Sep 1999)
ISSN: 0146-0404 [Print] United States |
PMID | 10476814
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Dipeptides
- N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide
- Protease Inhibitors
- Sodium Hydroxide
- Metalloendopeptidases
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Topics |
- Animals
- Burns, Chemical
(drug therapy, metabolism, pathology)
- Cornea
(drug effects, metabolism, pathology)
- Cytokines
(metabolism)
- Dipeptides
(pharmacology)
- Eye Burns
(chemically induced, metabolism, pathology)
- Female
- Metalloendopeptidases
(antagonists & inhibitors)
- Mice
- Mice, Inbred ICR
- Protease Inhibitors
(pharmacology)
- Sodium Hydroxide
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