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Effect of an aluminum hydroxide-magnesium hydroxide combination drug on adhesion, IL-8 inducibility, and expression of HSP60 by Helicobacter pylori.

AbstractBACKGROUND:
Co-magaldrox (Maalox) is used world-wide as an antacid and as a cytoprotective agent for gastritis and peptic ulcer diseases. We examined the effects of co-magaldrox on Helicobacter pylori.
METHODS:
Adhesion of H. pylori to human gastric epithelial cells (MKN45) was evaluated by flow cytometry. Morphologic changes in H. pylori caused by co-magaldrox were determined by scanning electron microscopy. Induction of interleukin-8 (IL-8) from MKN45 cells was examined with enzyme-linked immunosorbent assay, and the intracellular and extracellular expression of heat-shock protein 60 (HSP60) was analyzed with sodium dodecyl sulphate-polyacrylamide gel electrophoresis and flow cytometry.
RESULTS:
Adhesion of H. pylori to MKN 45 cells was significantly inhibited by 1.25%-5% comagaldrox. H. pylori aggregated with co-magaldrox according to an electron microscopic examination. IL-8 secretion from MKN45 cells after H. pylori infection was also inhibited by co-magaldrox. Extracellular expression of HSP60 on the surface of H. pylori was decreased after treatment with comagaldrox, whereas the intracellular synthesis of HSP60 was not. HSP60-induced IL-8 secretion was significantly inhibited by co-magaldrox in a dose-dependent manner.
CONCLUSIONS:
These results show that co-magaldrox suppressed the expression of the following virulence factors: adhesion, IL-8 inducibility, and expression of extracellular HSP60. Therefore, co-magaldrox is a potent anti-H. pylori and cytoprotective drug.
AuthorsS Kamiya, H Yamaguchi, T Osaki, H Taguchi, M Fukuda, H Kawakami, H Hirano
JournalScandinavian journal of gastroenterology (Scand J Gastroenterol) Vol. 34 Issue 7 Pg. 663-70 (Jul 1999) ISSN: 0036-5521 [Print] England
PMID10466876 (Publication Type: Journal Article)
Chemical References
  • Antacids
  • Chaperonin 60
  • Drug Combinations
  • Interleukin-8
  • aluminum hydroxide, magnesium hydroxide, drug combination
  • Aluminum Hydroxide
  • Magnesium Hydroxide
Topics
  • Aluminum Hydroxide (pharmacology)
  • Antacids (pharmacology)
  • Bacterial Adhesion (drug effects, physiology)
  • Cell Line
  • Chaperonin 60 (biosynthesis)
  • Drug Combinations
  • Electrophoresis, Polyacrylamide Gel
  • Flow Cytometry
  • Gastric Mucosa (microbiology, physiology)
  • Helicobacter pylori (drug effects, physiology, ultrastructure)
  • Humans
  • Immunoblotting
  • Interleukin-8 (metabolism)
  • Magnesium Hydroxide (pharmacology)
  • Microscopy, Electron, Scanning

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