Abstract |
The antitumor effect of the type I IFN, IFN-omega, was evaluated in both in vitro and in vivo studies of human cancer. For these studies, the cDNA for human IFN-omega was cloned into a eukaryotic expression plasmid DNA (pDNA) driven by the cytomegalovirus promoter. Supernatants from UM449 cells transfected in vitro with IFN-omega pDNA had antiproliferative effects on 11 of 13 human tumor cell lines. For in vivo studies, nude mice were implanted s.c. with one of the following human tumors: NIH: OVCAR-3 ovarian carcinoma, A375 melanoma, or A431 epidermoid carcinoma. Direct intratumoral injection of 100 microg of a IFN-omega pDNA DMRIE/DOPE complex (1:1 DNA: DMRIE mass ratio) for 6 consecutive days resulted in a significant reduction in the tumor volume of NIH: OVCAR-3 ovarian carcinoma or A375 melanoma (P = 0.02). IFN-omega pDNA delivered by i.m. injection also had an antitumor effect. Nude mice bearing s.c. A431 epidermoid carcinoma and injected i.m. with 100 microg of IFN-omega pDNA, twice per week for 3 weeks, had a significant reduction in tumor volume (P = 0.009). These results demonstrate for the first time that IFN-omega can have in vivo antitumor effects in several models of human cancer.
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Authors | H M Horton, P Hernandez, S E Parker, K M Barnhart |
Journal | Cancer research
(Cancer Res)
Vol. 59
Issue 16
Pg. 4064-8
(Aug 15 1999)
ISSN: 0008-5472 [Print] United States |
PMID | 10463608
(Publication Type: Journal Article)
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Chemical References |
- Interferon Type I
- interferon omega 1
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Topics |
- Animals
- Female
- Humans
- Interferon Type I
(administration & dosage, genetics)
- Melanoma
(drug therapy, genetics)
- Mice
- Mice, Nude
- Neoplasm Transplantation
- Ovarian Neoplasms
(drug therapy, genetics)
- Skin Neoplasms
(drug therapy, genetics)
- Transfection
- Transplantation, Heterologous
- Tumor Cells, Cultured
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