Intraventricular vascular endothelial growth factor antibody increases infarct volume following transient cerebral ischemia.

Abstract

AIM:

To clarify the role of vascular endothelial growth factor (VEGF) in neuronal damage induced by cerebral ischemia.

METHODS:

Expression of VEGF in adult rat brain was measured by immunohistochemistry. Transient middle cerebral artery occlusion (MCAO) model was induced by placing a nylon thread in the lumen of the internal carotid artery. The infarct volume was shown with 2,3,5-triphenyltetrazolium chloride (TTC) staining and quantitated by computer image analyzer with and without VEGF antibody treatment.

RESULTS:

VEGF expression was widely distributed in neuronal cells besides vascular endothelial cells, and the neuronal distribution of VEGF was specific. After intraventricular treatment with VEGF antibody (0.1 g.L-1 daily, for 7 d following the ischemia), infarct volume in the antibody treatment was increased versus vehicle-treated rats [(21.6 +/- 2.7 vs 16 +/- 6) mm3, P < 0.05] respectively.

CONCLUSION:

Intraventricular injection of VEGF antibody increased the infarct volume after focal cerebral ischemia in rats, suggesting that expression of neuronal VEGF may be one of neuronal protective mechanisms.

• Authors: W L Bao, S D Lu, H Wang, F Y Sun
• Journal: Zhongguo yao li xue bao = Acta pharmacologica Sinica (Zhongguo Yao Li Xue Bao) Vol. 20 Issue 4 Pg. 313-8 (Apr 1999) ISSN: 0253-9756 [Print] China
• PMID: 10452115 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• Endothelial Growth Factors
• Lymphokines
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors

Topics

• Animals
• Antibodies
• Brain (metabolism, pathology)
• Endothelial Growth Factors (biosynthesis, physiology)
• Ischemic Attack, Transient (metabolism, pathology)
• Lymphokines (biosynthesis, physiology)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors