Securinine induced apoptosis in human leukemia HL-60 cells.

Abstract

AIM:

To study whether securinine might induce apoptosis in human leukemia HL-60 cells.

METHODS:

Inhibition of proliferation was measured using MTT assay. The amount of apoptotic cells was measured by flow cytometry. DNA fragmentation was visualized by DNA agarose gel electrophoresis and the cellular changes were observed by electron microscope.

RESULTS:

Securinine 5-80 mg.L-1 elicited typical apoptosis morphological changes and DNA fragmentation in a concentration-dependent manner in HL-60 cells. Securinine inhibited HL-60 cell proliferation in a time- and concentration-dependent manner. The IC50 and 95% confidence limits were 27 (15-47) mg.L-1 after 12-h treatment with securinine.

CONCLUSION:

Securinine induced apoptosis in HL-60 cells.

• Authors: N Z Dong, Z L Gu, W H Chou, C Y Kwok
• Journal: Zhongguo yao li xue bao = Acta pharmacologica Sinica (Zhongguo Yao Li Xue Bao) Vol. 20 Issue 3 Pg. 267-70 (Mar 1999) ISSN: 0253-9756 [Print] China
• PMID: 10452105 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• Antineoplastic Agents, Phytogenic
• Azepines
• DNA, Neoplasm
• Heterocyclic Compounds, 4 or More Rings
• Heterocyclic Compounds, Bridged-Ring
• Lactones
• Piperidines
• securinine

Topics

• Alkaloids (pharmacology)
• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis (drug effects)
• Azepines
• Cell Division (drug effects)
• DNA Fragmentation
• DNA, Neoplasm (drug effects)
• Dose-Response Relationship, Drug
• HL-60 Cells (ultrastructure)
• Heterocyclic Compounds, 4 or More Rings
• Heterocyclic Compounds, Bridged-Ring
• Humans
• Lactones
• Piperidines