Treatment of experimental autoimmune arthritis by nasal administration of a type II collagen-cholera toxoid conjugate vaccine.

Abstract

OBJECTIVE:

To assess the efficacy of intranasal administration of microgram amounts of type II collagen (CII) coupled to cholera toxin B subunit (CTB) with respect to the development of collagen-induced arthritis, even when given after the onset of clinically evident arthropathy.

METHODS:

DBA/1 mice were immunized with CII to induce arthritis and were subsequently treated with CTB-CII, CTB-conjugated ovalbumin, or CII alone. The incidence and severity of arthritis were assessed clinically and histologically.

RESULTS:

Treatment with CTB-CII conjugate effectively suppressed leukocyte infiltration into the synovium and prevented bone erosion. Comparable doses of unconjugated CII administered by the same route were relatively ineffective. Protection with nasal CTB-CII vaccine was associated with decreased production of interleukin-4 (IL-4), IL-6, and interferon-gamma and with reduced CII-specific IgG1 and IgG2a antibody responses in regional lymph nodes.

CONCLUSION:

Nasal treatment with CTB-CII appears to result in decreased peripheral Th1 and Th2 responses to collagen. These results suggest that intranasal vaccination with CTB-CII may offer an effective immunotherapeutic means for the control of chronic polyarthritis.

• Authors: A Tarkowski, J B Sun, R Holmdahl, J Holmgren, C Czerkinsky
• Journal: Arthritis and rheumatism (Arthritis Rheum) Vol. 42 Issue 8 Pg. 1628-34 (Aug 1999) ISSN: 0004-3591 [Print] United States
• PMID: 10446861 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Autoantibodies
• Immunotoxins
• Vaccines
• Collagen
• Cholera Toxin

Topics

• Administration, Intranasal
• Animals
• Antibody Formation (immunology)
• Arthritis, Experimental (immunology, prevention & control)
• Autoantibodies (immunology)
• Autoimmune Diseases (prevention & control)
• Cholera Toxin (immunology)
• Collagen (immunology)
• Disease Progression
• Immunotoxins (immunology)
• Male
• Mice
• Mice, Inbred DBA
• Vaccines (administration & dosage)