Abstract |
E10-5A3 is a dhfr-ts- Leishmania major double knockout auxotrophic shown previously to induce substantial protection against virulent L. major infection in both genetically susceptible and resistant mice. We investigated the capacity of dhfr-ts- to protect against heterologous infection by L. amazonensis. The degree of protection was evaluated by immunization of BALB/c or C57BL/6 mice with E10-5A3, followed by L. amazonensis challenge. Whether immunized by subcutaneous (SC) or intravenous (IV) inoculation, susceptible and resistant mice displayed a partial degree of protection against challenge with virulent L. amazonensis. SC-immunized BALB/c mice developed lesions 40 to 65% smaller than non immunized mice, while IV immunization led to protection ranging from 40 to 75% in four out of six experiments compared to non immunized animals. The resistant C57BL/6 mice displayed comparable degrees of protection, 57% by SC and 49% by IV immunization. Results are encouraging as it has been previously difficult to obtain protection by SC vaccination against Leishmania, the preferred route for human immunization.
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Authors | P Veras, C Brodskyn, F Balestieri, L d Freitas, A Ramos, A Queiroz, A Barral, S Beverley, M Barral-Netto |
Journal | Memorias do Instituto Oswaldo Cruz
(Mem Inst Oswaldo Cruz)
1999 Jul-Aug
Vol. 94
Issue 4
Pg. 491-6
ISSN: 0074-0276 [Print] Brazil |
PMID | 10446007
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Protozoan
- Multienzyme Complexes
- Protozoan Vaccines
- thymidylate synthase-dihydrofolate reductase
- Tetrahydrofolate Dehydrogenase
- Thymidylate Synthase
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Topics |
- Animals
- Antigens, Protozoan
(administration & dosage)
- Leishmania major
(immunology)
- Leishmaniasis, Cutaneous
(immunology, prevention & control)
- Mice
- Mice, Inbred BALB C
- Mice, Knockout
- Mice, Mutant Strains
- Multienzyme Complexes
(immunology)
- Protozoan Vaccines
(immunology)
- Tetrahydrofolate Dehydrogenase
(immunology)
- Thymidylate Synthase
(immunology)
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