Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation.

Abstract	ATP-dependent nucleosome remodeling and core histone acetylation and deacetylation represent mechanisms to alter nucleosome structure. NuRD is a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. The histone deacetylases HDAC1 and HDAC2 and the histone binding proteins RbAp48 and RbAp46 form a core complex shared between NuRD and Sin3-histone deacetylase complexes. The histone deacetylase activity of the core complex is severely compromised. A novel polypeptide highly related to the metastasis-associated protein 1, MTA2, and the methyl-CpG-binding domain-containing protein, MBD3, were found to be subunits of the NuRD complex. MTA2 modulates the enzymatic activity of the histone deacetylase core complex. MBD3 mediates the association of MTA2 with the core histone deacetylase complex. MBD3 does not directly bind methylated DNA but is highly related to MBD2, a polypeptide that binds to methylated DNA and has been reported to possess demethylase activity. MBD2 interacts with the NuRD complex and directs the complex to methylated DNA. NuRD may provide a means of gene silencing by DNA methylation.
Authors	Y Zhang, H H Ng, H Erdjument-Bromage, P Tempst, A Bird, D Reinberg
Journal	Genes & development (Genes Dev) Vol. 13 Issue 15 Pg. 1924-35 (Aug 01 1999) ISSN: 0890-9369 [Print] United States
PMID	10444591 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References	Autoantigens CHD4 protein, human Carrier Proteins DNA-Binding Proteins MBD2 protein MBD3 protein, human Mbd3 protein, mouse Multienzyme Complexes Nuclear Proteins Nucleosomes RBBP4 protein, human RBBP7 protein, human Rbbp4 protein, mouse Recombinant Fusion Proteins Repressor Proteins Retinoblastoma-Binding Protein 4 Retinoblastoma-Binding Protein 7 Transcription Factors DNA MTA2 protein, human HDAC1 protein, human Hdac2 protein, mouse Histone Deacetylase 1 Histone Deacetylase 2 Histone Deacetylases Mi-2 Nucleosome Remodeling and Deacetylase Complex Adenosine Triphosphatases DNA Helicases
Topics	Adenosine Triphosphatases Amino Acid Sequence Autoantigens (isolation & purification, metabolism) Base Sequence Carrier Proteins (chemistry, genetics, isolation & purification, metabolism) Cloning, Molecular DNA (chemistry, genetics, metabolism) DNA Helicases DNA Methylation DNA-Binding Proteins (chemistry, genetics, isolation & purification, metabolism) HeLa Cells Histone Deacetylase 1 Histone Deacetylase 2 Histone Deacetylases (chemistry, genetics, isolation & purification, metabolism) Humans Mi-2 Nucleosome Remodeling and Deacetylase Complex Molecular Sequence Data Multienzyme Complexes (chemistry, genetics, isolation & purification, metabolism) Nuclear Proteins (isolation & purification, metabolism) Nucleosomes (chemistry, genetics, metabolism) Protein Binding Recombinant Fusion Proteins (isolation & purification, metabolism) Repressor Proteins Retinoblastoma-Binding Protein 4 Retinoblastoma-Binding Protein 7 Sequence Homology, Amino Acid Transcription Factors

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