HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Sigma competition: the contest between bacteriophage T4 middle and late transcription.

Abstract
In bacterial transcription, diverse sigma-family promoter recognition proteins compete for a common RNA polymerase core. Bacteriophage T4 infection ultimately reduces this competition to a duel between activated viral middle and enhanced late transcription, involving two sigma proteins, two phage-encoded activator proteins and two phage-specific co-activators. This competition has been analyzed in vitro, and the relative abundances in T4-infected Escherichia coli of the participating proteins have been measured. Activated late transcription holds an advantage over activated middle transcription, especially at higher ionic strength. This advantage is further compounded by ADP-ribosylation of the RNA polymerase alpha subunits, and by the phage-specific, RNA polymerase core-bound RpbA subunit. The largest contribution to the middle-late competition is made by gp55, the late sigma factor, but not enough of gp55 is produced during T4 infection to shut off middle transcription by direct competition with sigma(70). AsiA, the originally identified anti-sigma protein is not a major determinant of middle-late competition.
AuthorsS Kolesky, M Ouhammouch, E N Brody, E P Geiduschek
JournalJournal of molecular biology (J Mol Biol) Vol. 291 Issue 2 Pg. 267-81 (Aug 13 1999) ISSN: 0022-2836 [Print] Netherlands
PMID10438620 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
CopyrightCopyright 1999 Academic Press.
Chemical References
  • AsiA protein, Enterobacteria phage T4
  • DNA-Binding Proteins
  • MotA protein, Enterobacteria phage T4
  • Sigma Factor
  • Trans-Activators
  • Transcription Factors
  • Viral Proteins
  • gene 33 protein, Enterobacteria phage T4
  • gene 45 protein, Enterobacteria phage T4
  • gene 55 protein, Enterobacteria phage T4
  • gene 59 protein, Enterobacteria phage T4
  • RNA polymerase sigma 70
  • DNA-Directed RNA Polymerases
Topics
  • Animals
  • Bacteriophage T4 (genetics)
  • DNA-Binding Proteins (metabolism)
  • DNA-Directed RNA Polymerases (metabolism)
  • Osmolar Concentration
  • Promoter Regions, Genetic
  • Rabbits
  • Sigma Factor (metabolism)
  • Templates, Genetic
  • Trans-Activators (metabolism)
  • Transcription Factors (metabolism)
  • Transcription, Genetic
  • Transcriptional Activation
  • Viral Proteins (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: