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Effect of pH on Ki-67 antigen retrieval in renal cell carcinoma.

Abstract
The efficacy of Ki-67 antigen retrieval (AR) was assessed in 20 formalin fixed-paraffin embedded specimens of renal cell carcinoma using three solutions with different pH values. The AR was performed by means of microwave oven heating up to 97 degrees C for 15 minutes. Better results were obtained using solutions with pH values 8.0 and 9.9 compared with AR in a solution of pH 6.0, which is routinely used in daily pathological practice. Individual testing of various AR conditions is recommended to obtain the best immunohistochemical results.
AuthorsA Masera, O Stanisa, Z Ovcak, A Cör
JournalActa medica Croatica : casopis Hravatske akademije medicinskih znanosti (Acta Med Croatica) Vol. 53 Issue 1 Pg. 15-7 ( 1999) ISSN: 1330-0164 [Print] Croatia
PMID10437272 (Publication Type: Journal Article)
Chemical References
  • Ki-67 Antigen
Topics
  • Carcinoma, Renal Cell (immunology)
  • Humans
  • Hydrogen-Ion Concentration
  • Immunohistochemistry
  • Ki-67 Antigen (metabolism)
  • Kidney Neoplasms (immunology)

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