Genetic analysis of HLA- and HPA-typing in idiopathic (autoimmune) thrombocytopenic purpura patients treated with cepharanthin.

Abstract	We performed genetic analysis of human leukocyte antigen (HLA) and human platelet antigen (HPA) in 45 patients with cepharanthin-treated idiopathic thrombocytopenic purpura. HLA-typing was performed by the polymerase chain reaction-restriction fragment length polymorphism method, and HPA-typing by a polymerase chain reaction-sequence-specific primer method. There were 14 responders and 31 nonresponders. Responders included many patients who had already been treated with prednisolone. HLA-DRB10901 was significantly more common in responders than in nonresponders. In contrast, HLA-DRB10410 and DQB1*0401 were significantly more common in nonresponders. The a/b genotype of HPA-2a/2a (Ko(b)/Ko(b)) was significantly increased in responders. In contrast, HPA-2a/2b (Ko(b)/Ko(a)) and HPA-3a/3b (Bak(a)/Bak(b)) were significantly more common in nonresponders. These findings suggest that genetic studies of HLA and HPA can predict the response of idiopathic thrombocytopenic purpura to cepharanthin.
Authors	S Nomura, T Matsuzaki, M Yamaoka, Y Ozaki, M Nagahama, C Yoshimura, H Kagawa, S Nakayama, S Fukuhara
Journal	Autoimmunity (Autoimmunity) Vol. 30 Issue 2 Pg. 99-105 ( 1999) ISSN: 0891-6934 [Print] England
PMID	10435723 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	2a alloantigen, human 3a alloantigen, human Alkaloids Anti-Inflammatory Agents Anti-Inflammatory Agents, Non-Steroidal Antigens, Human Platelet Autoantigens Benzylisoquinolines HLA Antigens HLA-DQ Antigens HLA-DQ beta-Chains HLA-DQB1 antigen HLA-DR Antigens HLA-DRB1 Chains cepharanthine Prednisolone
Topics	Adult Aged Alkaloids (pharmacology, therapeutic use) Anti-Inflammatory Agents (therapeutic use) Anti-Inflammatory Agents, Non-Steroidal (pharmacology, therapeutic use) Antigens, Human Platelet (analysis, genetics) Autoantigens (genetics, immunology) Autoimmune Diseases (drug therapy, genetics, immunology, therapy) Benzylisoquinolines Blood Platelets (immunology) Combined Modality Therapy Drug Resistance (genetics) Endocytosis (drug effects) Female Genetic Predisposition to Disease Genotype HLA Antigens (analysis, genetics) HLA-DQ Antigens (analysis, genetics) HLA-DQ beta-Chains HLA-DR Antigens (analysis, genetics) HLA-DRB1 Chains Humans Male Middle Aged Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Polymorphism, Single-Stranded Conformational Prednisolone (therapeutic use) Purpura, Thrombocytopenic, Idiopathic (drug therapy, genetics, immunology, therapy) Splenectomy Treatment Outcome

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