Labeled carcinoembryonic antigen antibodies excitable by infrared rays: a novel diagnostic method for micro cancers in the digestive tract.

Abstract

OBJECT:

An indocyanine green derivative (ICG-sulfo-OSu) was used as the labeling substance for monoclonal antibody, and a fluorescence imaging system appropriate for ICG-sulfo-OSu excitable by infrared rays (IR) was developed. The goal of this study was to demonstrate antibody labeling at the tissue level using this new imaging system.

MATERIALS AND METHODS:

ICG-sulfo-OSu labeled mouse anti-human carcinoembryonic antigen (CEA) monoclonal antibody, a newly developed imaging system, and an infrared ray microscope were employed in this experiment. Paraffin sections of human colon cancer previously proven to have cross-reactivity to anti-CEA antibody were examined.

RESULTS:

Positive staining was seen as a brownish discoloration of oxidized 3,3'-diaminobenzidine tetrahydrochloride (DAB) in sections that reacted with ICG-sulfo-OSu-labeled anti-CEA antibody, and the fluorescence was well-matched with the oxidized DAB-positive sites.

CONCLUSION:

Specific antibodies labeled with ICG-sulfo-OSu have significant affinity to cancer cells and seem to reflect sufficient amounts of fluorescence by IR to be useful in a system for the endoscopic detection of micro cancers using the immunohistochemical staining method.

• Authors: N Muguruma, S Ito, T Bando, S Taoka, Y Kusaka, S Hayashi, S Ichikawa, Y Matsunaga, Y Tada, S Okamura, K Ii, K Imaizumi, K Nakamura, K Takesako, S Shibamura
• Journal: Internal medicine (Tokyo, Japan) (Intern Med) Vol. 38 Issue 7 Pg. 537-42 (Jul 1999) ISSN: 0918-2918 [Print] Japan
• PMID: 10435358 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Carcinoembryonic Antigen
• Fluorescent Dyes
• indocyanine green-sulfo-OSu
• Indocyanine Green

Topics

• Adenocarcinoma (diagnosis, immunology)
• Animals
• Antibodies, Monoclonal
• Carcinoembryonic Antigen (metabolism)
• Colonic Neoplasms (diagnosis, immunology)
• Cross Reactions
• Fluorescent Dyes
• Humans
• Immunohistochemistry
• Indocyanine Green (analogs & derivatives)
• Infrared Rays
• Mice
• Microscopy, Fluorescence (instrumentation)
• Paraffin Embedding