HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

The macromolecular characteristics of cartilage proteoglycans do not change when synthesis is up-regulated by link protein peptide.

Abstract
Previous studies have shown that a synthetic, unglycosylated analogue of the N-terminal peptide from link protein can function as a growth factor and up-regulate proteoglycan biosynthesis in explant cultures of normal human articular cartilage from a wide age range of subjects (McKenna et al., Arthritis Rheum. 41 (1998) 157-162). The present work further shows that link peptide increased proteoglycan synthesis by cartilage cultured in both the presence and absence of serum, suggesting that the mechanism of up-regulation may be different from that of insulin-like growth factors. The proteoglycans synthesised during stimulation with link peptide were of normal hydrodynamic size and the ratio of core protein to glycosaminoglycan side chains and the proportions of the large proteoglycan aggrecan to the small proteoglycans, decorin and biglycan, remained constant. Aggrecan molecules were equally capable of forming aggregates as those from control tissues and the relative proportions of decorin and biglycan were unchanged showing that both were co-ordinately up-regulated. These results confirmed that this novel peptide is a potent stimulator of proteoglycan synthesis by articular cartilage and showed that the newly synthesised proteoglycans were of normal composition.
AuthorsH Liu, L A McKenna, M F Dean
JournalBiochimica et biophysica acta (Biochim Biophys Acta) Vol. 1428 Issue 2-3 Pg. 191-200 (Aug 5 1999) ISSN: 0006-3002 [Print] NETHERLANDS
PMID10434036 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Aggrecans
  • BGN protein, human
  • Biglycan
  • DCN protein, human
  • Decorin
  • Extracellular Matrix Proteins
  • Lectins, C-Type
  • Proteins
  • Proteoglycans
  • Sulfur Radioisotopes
  • link protein
  • Tritium
  • Papain
  • Chondroitinases and Chondroitin Lyases
Topics
  • Adolescent
  • Adult
  • Aggrecans
  • Amino Acid Sequence
  • Biglycan
  • Cartilage, Articular (drug effects, metabolism)
  • Chondroitinases and Chondroitin Lyases
  • Decorin
  • Extracellular Matrix Proteins
  • Female
  • Humans
  • Lectins, C-Type
  • Male
  • Molecular Sequence Data
  • Molecular Weight
  • Papain
  • Proteins (chemistry, pharmacology)
  • Proteoglycans (biosynthesis, chemistry)
  • Sulfur Radioisotopes
  • Tritium
  • Up-Regulation

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: