Quantification of lamivudine (3TC) by competitive immunoassay.

Lamivudine or 3TC, the (-) eniantiomer of 2'-deoxy-3'-thiacytidine, is a prototype of a novel class of levogyre dideoxynucleosides analogues used in treatment of HIV and HBV infection. We describe a method corresponding to the first enzyme immunoassay for quantifying this antiviral drug. This technique use an enzyme conjugate that not require the use of radioactive labelling. In this study, anti-3TC antibodies were raised in rabbits by immunising with 3TC-HS-kelhoyle limpet hemocyanin (KLH) conjugate.
AuthorsF Akeb, C Creminon, M C Nevers, J Grassi, D Duval, R Guedj
JournalNucleosides & nucleotides (Nucleosides Nucleotides) 1999 Apr-May Vol. 18 Issue 4-5 Pg. 893-6 ISSN: 0732-8311 [Print] UNITED STATES
PMID10432705 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anti-HIV Agents
  • Reverse Transcriptase Inhibitors
  • Lamivudine
  • Anti-HIV Agents (analysis, immunology)
  • Cross Reactions
  • Immunoassay (methods)
  • Lamivudine (analysis, immunology)
  • Reverse Transcriptase Inhibitors (analysis, immunology)

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