Abstract |
Lamivudine or 3TC, the (-) eniantiomer of 2'-deoxy-3'-thiacytidine, is a prototype of a novel class of levogyre dideoxynucleosides analogues used in treatment of HIV and HBV infection. We describe a method corresponding to the first enzyme immunoassay for quantifying this antiviral drug. This technique use an enzyme conjugate that not require the use of radioactive labelling. In this study, anti-3TC antibodies were raised in rabbits by immunising with 3TC-HS-kelhoyle limpet hemocyanin (KLH) conjugate.
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Authors | F Akeb, C Creminon, M C Nevers, J Grassi, D Duval, R Guedj |
Journal | Nucleosides & nucleotides
(Nucleosides Nucleotides)
1999 Apr-May
Vol. 18
Issue 4-5
Pg. 893-6
ISSN: 0732-8311 [Print] United States |
PMID | 10432705
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-HIV Agents
- Reverse Transcriptase Inhibitors
- Lamivudine
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Topics |
- Anti-HIV Agents
(analysis, immunology)
- Cross Reactions
- Immunoassay
(methods)
- Lamivudine
(analysis, immunology)
- Reverse Transcriptase Inhibitors
(analysis, immunology)
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