HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Determination of the sum of bilirubin sugar conjugates in plasma by bilirubin oxidase.

AbstractBACKGROUND:
A reliable indicator of cholestasis is the presence of abnormal concentrations of bilirubin mono- and diglucuronide [conjugated bilirubin (CB)] in blood. A routine assay of CB is available only to those who possess a certain type of clinical analyzer. We describe a two-point manual method for CB that could be adapted as a rate assay to automated clinical analyzers.
METHODS:
The measurement of CB is based on its oxidation to biliverdin by bilirubin oxidase. The resulting decrease in absorbance at 460 nm is proportional to the CB concentration. The assay is calibrated with solutions of ditaurobilirubin in human serum.
RESULTS:
Under the conditions of the assay (0.1 mol/L glycine buffer, pH 10.0; reaction time, 2 min), only 5% of unconjugated bilirubin is oxidized and delta-bilirubin is not oxidized at all. Results obtained with the bilirubin oxidase method agreed well with those obtained by HPLC. The long-term CVs at CB concentrations of 6 and 63.4 mg/L were 20% and 2.6%, respectively. The reference values, established by analyzing 51 plasma specimens from healthy adults, were 0.0-1.2 mg/L, with a mean value of 0.2 mg/L.
CONCLUSIONS:
The proposed method for CB has good analytical specificity and obviates the requirement for HPLC or a dry chemistry analyzer. The measurement of CB in blood is superior to the measurement of direct bilirubin because an abnormal concentration of direct bilirubin does not necessarily indicate the presence of cholestasis.
AuthorsB T Doumas, F Yein, B Perry, B Jendrzejczak, A Kessner
JournalClinical chemistry (Clin Chem) Vol. 45 Issue 8 Pt 1 Pg. 1255-60 (Aug 1999) ISSN: 0009-9147 [Print] England
PMID10430792 (Publication Type: Journal Article)
Chemical References
  • Carbohydrates
  • Oxidoreductases
  • Oxidoreductases Acting on CH-CH Group Donors
  • bilirubin oxidase
  • Bilirubin
Topics
  • Bilirubin (blood, chemistry)
  • Carbohydrates (blood, chemistry)
  • Chromatography, High Pressure Liquid
  • Humans
  • Jaundice (blood)
  • Kinetics
  • Oxidoreductases (chemistry)
  • Oxidoreductases Acting on CH-CH Group Donors
  • Reference Values
  • Sensitivity and Specificity

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: