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Relationship between cell migration and cell cycle during the initiation of epithelial to fibroblastoid transition.

Abstract
The NBT-II rat bladder carcinoma cell line, which displays epithelial to mesenchymal transition or EMT in response to FGF-1 stimulation, was used to study the interrelationships between cell cycle and cell scattering and locomotion. Time-lapse video microscopy experiments were performed with asynchronous growing cells and lovastatin-arrested cells. FGF-1 stimulation induced cell movement in cells in all phases of the cell cycle, except G2 + M phase, in which cells did not respond to stimulation. The delay between cell stimulation and cell movement depended on the age of the cell at the beginning of cell stimulation: cells less than 4 h old when stimulated by FGF-1 had a 1-h delay whereas cells more than 4 h old had a 3-h delay. Cells stimulated before they were 4 h old were temporarily arrested in their cell cycle progression. Older cells underwent mitosis on schedule. Lovastatin-treated cells were shown to be synchronized in the G1 phase and to migrate simultaneously after FGF-1 stimulation. These results indicate that the G1 phase was a critical phase for FGF-1 induced cell migration during epithelial to fibroblastoid transition.
AuthorsC Bonneton, J B Sibarita, J P Thiery
JournalCell motility and the cytoskeleton (Cell Motil Cytoskeleton) Vol. 43 Issue 4 Pg. 288-95 ( 1999) ISSN: 0886-1544 [Print] United States
PMID10423270 (Publication Type: Journal Article)
CopyrightCopyright 1999 Wiley-Liss, Inc.
Chemical References
  • Fibroblast Growth Factor 2
  • Fibroblast Growth Factor 1
Topics
  • Animals
  • Cell Cycle
  • Cell Movement
  • Epithelial Cells (drug effects)
  • Fibroblast Growth Factor 1
  • Fibroblast Growth Factor 2 (pharmacology)
  • Flow Cytometry
  • G1 Phase
  • Humans
  • Rats
  • Time Factors
  • Tumor Cells, Cultured
  • Videotape Recording

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