Abstract |
The NBT-II rat bladder carcinoma cell line, which displays epithelial to mesenchymal transition or EMT in response to FGF-1 stimulation, was used to study the interrelationships between cell cycle and cell scattering and locomotion. Time-lapse video microscopy experiments were performed with asynchronous growing cells and lovastatin-arrested cells. FGF-1 stimulation induced cell movement in cells in all phases of the cell cycle, except G2 + M phase, in which cells did not respond to stimulation. The delay between cell stimulation and cell movement depended on the age of the cell at the beginning of cell stimulation: cells less than 4 h old when stimulated by FGF-1 had a 1-h delay whereas cells more than 4 h old had a 3-h delay. Cells stimulated before they were 4 h old were temporarily arrested in their cell cycle progression. Older cells underwent mitosis on schedule. Lovastatin-treated cells were shown to be synchronized in the G1 phase and to migrate simultaneously after FGF-1 stimulation. These results indicate that the G1 phase was a critical phase for FGF-1 induced cell migration during epithelial to fibroblastoid transition.
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Authors | C Bonneton, J B Sibarita, J P Thiery |
Journal | Cell motility and the cytoskeleton
(Cell Motil Cytoskeleton)
Vol. 43
Issue 4
Pg. 288-95
( 1999)
ISSN: 0886-1544 [Print] United States |
PMID | 10423270
(Publication Type: Journal Article)
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Copyright | Copyright 1999 Wiley-Liss, Inc. |
Chemical References |
- Fibroblast Growth Factor 2
- Fibroblast Growth Factor 1
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Topics |
- Animals
- Cell Cycle
- Cell Movement
- Epithelial Cells
(drug effects)
- Fibroblast Growth Factor 1
- Fibroblast Growth Factor 2
(pharmacology)
- Flow Cytometry
- G1 Phase
- Humans
- Rats
- Time Factors
- Tumor Cells, Cultured
- Videotape Recording
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