HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Nucleotide sequence of thymidine kinase gene of sequential acyclovir-resistant herpes simplex virus type 1 isolates recovered from a child with Wiskott-Aldrich syndrome: evidence for reactivation of acyclovir-resistant herpes simplex virus.

Abstract
Recurrent acyclovir (ACV)-resistant (ACV-r) herpes simplex virus type 1 (HSV-1) infections occurred in a patient with Wiskott-Aldrich syndrome, an X-linked recessive immunodeficiency syndrome composed of three clinical characteristics of immunodeficiency, thrombocytopenia, and an eczematous dermatitis. The patient had severe and recurrent ACV-r herpes simplex and was treated with vidarabine in a satisfactory manner from 1993 to 1997. During the 4-year observation period, two ACV-sensitive (ACV-s) HSV-1 isolates and five ACV-r HSV-1 isolates were recovered. The nucleotide sequence of the thymidine kinase (TK) gene from these sequential ACV-r isolates was compared with the ACV-s isolates. A single nucleotide deletion of cytosine (C) from homopolymer stretch of four C residues between nucleotide 1061 and 1064 of the open reading frame was found in all ACV-r isolates. No other differences were observed in the TK nucleotide sequence between ACV-s and ACV-r isolates. The TK nucleotide sequences of the two ACV-s isolates were identical to each other and those of the five ACV-r isolates were identical to one another. These results suggest that the ACV-r HSV-1 might have derived from the ACV-s strain in the patient body and that TK-associated ACV-r HSV-1 can reactivate from latency.
AuthorsM Saijo, T Suzutani, K Itoh, Y Hirano, K Murono, M Nagamine, K Mizuta, M Niikura, S Morikawa
JournalJournal of medical virology (J Med Virol) Vol. 58 Issue 4 Pg. 387-93 (Aug 1999) ISSN: 0146-6615 [Print] United States
PMID10421406 (Publication Type: Case Reports, Journal Article)
Chemical References
  • Antiviral Agents
  • DNA, Viral
  • Thymidine Kinase
  • Acyclovir
Topics
  • Acyclovir (therapeutic use)
  • Amino Acid Sequence
  • Antiviral Agents (therapeutic use)
  • Base Sequence
  • Child
  • DNA, Viral (chemistry, genetics)
  • Drug Resistance, Microbial
  • Follow-Up Studies
  • Genetic Variation
  • Herpes Simplex (complications, virology)
  • Herpesvirus 1, Human (drug effects, genetics, isolation & purification)
  • Humans
  • Japan
  • Male
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Phosphorylation
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Thymidine Kinase (genetics)
  • Wiskott-Aldrich Syndrome (complications)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: