Abstract | OBJECTIVES: DESIGN: METHODS: CD34+ stem cells were exposed with M-tropic virus Ba-L or T-tropic viruses IIIB or Rut at day 1. Beta-chemokines were added to some cells before the virus and kept throughout the culture. Virus replication was measured throughout the maturation of these cells into CD1a+ DC and CD1a- CD14+ cells using enzyme-linked immunosorbent assay (ELISA) for p24, nested polymerase chain reaction (PCR) for env and intracellular p24 detection by flow cytometry. RESULTS: First, CD34+ stem cells acquired or were infected by live virus because maturing cells showed infection by both M- and T-tropic viruses. Second, the viruses replicated actively during the maturation of CD34+ stem cells toward CD1a+ DC and CD1a- CD14+ cells. Third, beta-chemokines suppressed infection by M-tropic virus Ba-L. And finally, beta-chemokines enhanced infection by T-tropic viruses IIIB and Rut. CONCLUSIONS: In addition to the initial anti-M-tropic virus effect by beta-chemokines, selective pressure on viruses may also result because of an increase in susceptibility to T-tropic virus. Caution should be taken when evaluating the effect of beta-chemokine receptor agonists in AIDS therapy.
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Authors | H Wang, N J English, C D Reid, J E Merson, S C Knight |
Journal | Journal of acquired immune deficiency syndromes (1999)
(J Acquir Immune Defic Syndr)
Vol. 21
Issue 3
Pg. 179-88
(Jul 01 1999)
ISSN: 1525-4135 [Print] United States |
PMID | 10421240
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, CD34
- Chemokine CCL3
- Chemokine CCL4
- Chemokines, CC
- Macrophage Inflammatory Proteins
- Receptors, CCR5
- Receptors, CXCR4
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Topics |
- Antigens, CD34
- Cell Count
- Chemokine CCL3
- Chemokine CCL4
- Chemokines, CC
(physiology)
- Dendritic Cells
(virology)
- Gene Expression
- HIV-1
(pathogenicity)
- Hematopoietic Stem Cells
(virology)
- Humans
- Macrophage Inflammatory Proteins
(physiology)
- Macrophages
(virology)
- Receptors, CCR5
(genetics)
- Receptors, CXCR4
(genetics)
- T-Lymphocytes
(virology)
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