Abstract | BACKGROUND: METHODS: A PVG to ACI functional heterotopic rat heart model was used. Donor hearts were treated with either saline or AS-ICAM-1 ODN and 5 atm of hyperbaric pressure for 45 minutes. Anti-LFA-1 mAb was administered systemically prior to reperfusion of the allograft. Allografts were procured 24 hours after transplantation for assessment of reperfusion injury or 72 hours to determine ICAM-1 protein expression. RESULTS: Ex vivo administration of AS-ICAM-1 ODN led to decreases in percentage wet weight (77.1+/-0.83% vs 78.7+/-1.0%, p < 0.05), myeloperoxidase activity (3.14+/-0.72 vs 4.07+/-0.59, p < 0.05), contraction band necrosis (6.4+/-6.47% vs 21.1+/-7.43%, p < 0.01), and ICAM-1 protein expression determined by immunohistochemistry compared to saline controls. Treatment with anti-LFA-1 mAb resulted in decreases in wet weight ratio (76.7+/-0.63%, p < 0.05 vs saline), myeloperoxidase activity (3.58+/-0.39, p < 0.05 vs saline) and contraction band necrosis (11.8+/-3.56%, p < 0.05 vs saline). Combination of pressure administration of AS-ICAM-1 ODN and anti-LFA-1 mAb decreased wet weight ratios (77.1+/-0.93%, p < 0.05 vs saline), myeloperoxidase activity (2.88+/-0.44, p < 0.01 vs saline), and contraction band necrosis (6.75+/-5.67%, p < 0.05 vs saline). CONCLUSIONS:
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Authors | B T Feeley, A K Park, S Alexopoulos, E G Hoyt, M P Ennen, R S Poston Jr, R C Robbins |
Journal | The Annals of thoracic surgery
(Ann Thorac Surg)
Vol. 68
Issue 1
Pg. 119-24
(Jul 1999)
ISSN: 0003-4975 [Print] Netherlands |
PMID | 10421126
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- AS-ICAM-1 ODN
- Antibodies, Monoclonal
- Lymphocyte Function-Associated Antigen-1
- Oligodeoxyribonucleotides
- Oligodeoxyribonucleotides, Antisense
- Intercellular Adhesion Molecule-1
- Peroxidase
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Topics |
- Animals
- Antibodies, Monoclonal
(administration & dosage)
- Atmospheric Pressure
- Heart Transplantation
- Intercellular Adhesion Molecule-1
(analysis)
- Lymphocyte Function-Associated Antigen-1
(immunology)
- Male
- Myocardial Reperfusion Injury
(metabolism, pathology, prevention & control)
- Myocardium
(metabolism, pathology)
- Necrosis
- Oligodeoxyribonucleotides
- Oligodeoxyribonucleotides, Antisense
(administration & dosage)
- Peroxidase
(metabolism)
- Rats
- Rats, Inbred ACI
- Rats, Inbred Strains
- Transplantation, Heterotopic
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