Study of HLA class I restriction and the directed antigens of cytotoxic T lymphocytes at the tumor sites of ovarian cancer.

Abstract

The molecular basis of T-cell-mediated recognition of ovarian cancer cells remains to be fully addressed. In this study we investigated HLA class I restriction and directed antigens of cytotoxic T lymphocytes (CTL) at the sites of ovarian cancer. Three HLA-class-I-restricted CTL lines were established from the tumor sites of ovarian cancer by culturing tumor-infiltrating lymphocytes or tumor-associated ascitic lymphocytes with interleukin-2: (1) HLA-A2402-restricted and ovarian-adenocarcinoma-specific CTL, (2) HLA-A2-restricted CTL recognizing histologically different cancers, and (3) HLA-B52-restricted and ovarian-cancer-specific CTL. HLA-A0201, HLA-A0206 and HLA-A0207 tumor cells were lysed by the HLA-A2-restricted CTL. HLA-B52 restriction of the third CTL line was confirmed by the transfection of HLA-B5201 cDNA into the tumor cells. The HLA-A2-restricted CTL recognized the SART-1, but not the MAGE-1 or MAGE-3 antigen. These results may facilitate a better understanding of the molecular basis of tumor-specific immunity at the tumor site of ovarian cancer.

• Authors: A Yamada, K Kawano, N Harashima, F Niiya, K Nagai, T Kobayashi, T Mine, K Ushijima, T Nishida, K Itoh
• Journal: Cancer immunology, immunotherapy : CII (Cancer Immunol Immunother) 1999 May-Jun Vol. 48 Issue 2-3 Pg. 147-52 ISSN: 0340-7004 [Print] Germany
• PMID: 10414469 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Histocompatibility Antigens Class I
• MAGEA1 protein, human
• MAGEA3 protein, human
• Melanoma-Specific Antigens
• Neoplasm Proteins
• Ribonucleoproteins, Small Nuclear
• SART1 protein, human
• Interferon-gamma

Topics

• Animals
• Antigens, Neoplasm (immunology)
• COS Cells
• Female
• Histocompatibility Antigens Class I (physiology)
• Humans
• Interferon-gamma (biosynthesis)
• Melanoma-Specific Antigens
• Neoplasm Proteins (immunology)
• Ovarian Neoplasms (immunology)
• Ribonucleoproteins, Small Nuclear
• T-Lymphocytes, Cytotoxic (immunology)
• Tumor Cells, Cultured