Abstract |
Dysfunction of the cadherin- catenin complex, a key component of adherens junctions, is thought to confer invasive potential to cells. The aim of this study is to examine the expression and function of the E-cadherin/ catenin complex in gastric carcinoma cell lines. Expression of E-cadherin, alpha, beta and gamma-catenin and p120ctn, and of the adenomatous polyposis coli protein (APC), together with function of the cadherin- catenin complex was examined in a panel of gastric carcinoma cell lines, using immunocytochemistry, Western blotting and a cell-cell aggregation assay. Protein interactions were examined by sequential immunoprecipitation and immunoblotting with antibodies to E-cadherin, alpha, beta and gamma-catenin, p120ctn and APC. Abnormalities of E-cadherin, alpha- and beta-catenin expression, were associated with disturbance of E-cadherin- catenin complex composition, loss of membranous localization and loss of calcium-dependent aggregation in six gastric carcinoma cell lines. APC protein expression and interaction with beta-catenin was preserved in five cell lines. We demonstrate frequent abnormalities of expression and function of E-cadherin and catenins, and associated disturbance of E-cadherin-mediated intercellular adhesion in gastric carcinoma cell lines. These findings support the tumour suppressor role of the E-cadherin and its contribution to the development and progression of the neoplastic phenotype in gastric carcinoma.
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Authors | A U Jawhari, M Noda, M J Farthing, M Pignatelli |
Journal | British journal of cancer
(Br J Cancer)
Vol. 80
Issue 3-4
Pg. 322-30
(May 1999)
ISSN: 0007-0920 [Print] England |
PMID | 10408833
(Publication Type: Journal Article)
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Chemical References |
- CTNNA1 protein, human
- CTNNB1 protein, human
- CTNNB1 protein, mouse
- Cadherins
- Ctnna1 protein, mouse
- Cytoskeletal Proteins
- Macromolecular Substances
- Trans-Activators
- alpha Catenin
- beta Catenin
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Topics |
- Animals
- Cadherins
(biosynthesis, metabolism, physiology)
- Carcinoma
(metabolism, pathology)
- Cell Adhesion
(physiology)
- Cell Aggregation
(physiology)
- Cell Communication
(physiology)
- Cytoskeletal Proteins
(biosynthesis, metabolism, physiology)
- Humans
- Macromolecular Substances
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Stomach Neoplasms
(metabolism, pathology)
- Trans-Activators
- Tumor Cells, Cultured
- alpha Catenin
- beta Catenin
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