In vitro anticomplementary activity of hederagenin saponins isolated from roots of Dipsacus asper.

Abstract

Anticomplementary activity of hederagenin and related saponins isolated from Dipsacus asper was investigated in vitro. HN saponin F (3) was most potent with IC50 value of 3.7x10(-5) M followed by 3-O-beta-D-glucopyranosyl-(1->3)-alpha-L-rhamnopyranosyl-(1->2)-beta-L-+ ++arabi nopyranosyl hederagenin 28-O-beta-D-glucopyranosyl-(1->6)-beta-D-glucopyrano side (8), 3-O-beta-L-arabinopyranosyl hederagenin 28-O-beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranoside (5), dipsacus saponin A (4), and hederagenin (1) on the classical pathway (CP) of complement system, while the saponins 3-5 did not show the inhibition of hemolysis and rather increase the hemolysis on the alternative pathway (AP). However, all of C-3 monodesmosides [prosapogenin CP (2), dipsacus saponin B (6), and dipsacus saponin C (7)] evoked hemolysis directly on the erythrocytes.

• Authors: S R Oh, K Y Jung, K H Son, S H Park, I S Lee, K S Ahn, H K Lee
• Journal: Archives of pharmacal research (Arch Pharm Res) Vol. 22 Issue 3 Pg. 317-9 (Jun 1999) ISSN: 0253-6269 [Print] Korea (South)
• PMID: 10403139 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Complement Inactivator Proteins
• Plant Extracts
• Saponins
• Steroids
• Oleanolic Acid
• hederagenin

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Complement Hemolytic Activity Assay (methods)
• Complement Inactivator Proteins (pharmacology)
• Hemolysis (drug effects)
• Humans
• In Vitro Techniques
• Oleanolic Acid (analogs & derivatives, immunology)
• Plant Extracts (pharmacology)
• Saponins (immunology)
• Sheep
• Steroids