A Saprolegnia parasitica challenge system for rainbow trout: assessment of Pyceze as an anti-fungal agent for both fish and ova.

Abstract

A reproducible Saprolegnia parasitica spore delivery system was developed and demonstrated to be effective in providing a sustained spore challenge for up to 10 d. Treatment of rainbow trout with slow-release intraperitoneal implants containing cortisol resulted in chronically elevated blood cortisol levels and rendered the fish susceptible to infection by S. parasitica when exposed to the spore challenge. Sham-implanted fish were not susceptible to infection. Bronopol (2-bromo-2-nitro-propane-1,3-diol), formulated as Pyceze, was effective in protecting predisposed fish from infection by S. parasitica when administered as a daily bath/flush treatment at concentrations of 15 mg l-1 and greater. Pyceze was also demonstrated to protect fertilised rainbow trout ova from S. parasitica challenge when administered as a daily bath/flush treatment at concentrations of between 30 and 100 mg l-1. Pyceze appears to qualify as a safe and effective replacement for malachite green and formalin in the prevention of fungal infections in the aquaculture environment.

• Authors: T G Pottinger, J G Day
• Journal: Diseases of aquatic organisms (Dis Aquat Organ) Vol. 36 Issue 2 Pg. 129-41 (May 12 1999) ISSN: 0177-5103 [Print] Germany
• PMID: 10399041 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Bacterial Agents
• Drug Implants
• Immunosuppressive Agents
• Propylene Glycols
• Water
• Testosterone
• bronopol
• Hydrocortisone

Topics

• Animals
• Anti-Bacterial Agents (analysis, pharmacology, therapeutic use)
• Dose-Response Relationship, Drug
• Drug Implants
• Fish Diseases (drug therapy, microbiology)
• Hydrocortisone (administration & dosage, blood, therapeutic use)
• Immunosuppressive Agents (administration & dosage, therapeutic use)
• Infections (drug therapy, microbiology, veterinary)
• Oncorhynchus mykiss
• Oomycetes (drug effects, physiology)
• Propylene Glycols (analysis, pharmacology, therapeutic use)
• Spores (drug effects)
• Testosterone (administration & dosage, therapeutic use)
• Water (analysis)