Further characterization of the human clear cell sarcoma cell line HS-MM demonstrating a specific t(12;22)(q13;q12) translocation and hybrid EWS/ATF-1 transcript.

Abstract

Only a small number of clear cell sarcoma (CCS) cell lines have been reported, including the cell line HS-MM. In the present study, this cell line, maintained for more than 4 years since establishment, was further characterized by cytogenetic studies, fluorescence in situ hybridization (FISH) analysis, and reverse transcriptase-polymerase chain reaction (RT-PCR). HS-MM cells both in vitro and in vivo exhibited pseudodiploid karyotypes with the specific t(12;22)(q13;q12) translocation. The translocation between chromosomes 12 and 22 was confirmed by FISH analysis and the hybrid EWS/ATF-1 transcript induced by this translocation was detected by RT-PCR. The HS-MM cell line will be useful for further studies of CCS.

• Authors: H Sonobe, T Takeuchi, T Taguchi, K Shimizu, J Iwata, M Furihata, Y Ohtsuki
• Journal: The Journal of pathology (J Pathol) Vol. 187 Issue 5 Pg. 594-7 (Apr 1999) ISSN: 0022-3417 [Print] England
• PMID: 10398127 (Publication Type: Journal Article)
• Copyright: Copyright 1999 John Wiley & Sons, Ltd.

Chemical References

• EWS-ATF1 fusion protein, human
• Oncogene Proteins, Fusion
• Transcription Factors

Topics

• Chromosome Aberrations
• Chromosomes, Human, Pair 12
• Chromosomes, Human, Pair 22
• Humans
• In Situ Hybridization, Fluorescence
• Karyotyping
• Oncogene Proteins, Fusion (genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Sarcoma, Clear Cell (genetics, pathology)
• Transcription Factors (genetics)
• Translocation, Genetic
• Tumor Cells, Cultured