Identification of phosphate-regulated genes by differential expression in the UV-irradiated host system.

Abstract	The UV-irradiated host system has been used for identifying protein products of genes cloned in a phage vector. By starving the host cells for phosphate immediately before UV-irradiation, we demonstrate that phosphate-regulated genes can be easily identified. By employing this new technique, we also provide evidence showing that the gpsA gene might be a new member of the phosphate starvation-inducible (psi) genes of E. coli.
Authors	S E Chuang, F R Blattner
Journal	Journal of microbiological methods (J Microbiol Methods) Vol. 37 Issue 1 Pg. 93-6 (Jul 1999) ISSN: 0167-7012 [Print] Netherlands
PMID	10395468 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Bacterial Proteins Culture Media Phosphates PhoB protein, Bacteria Glycerolphosphate Dehydrogenase glycerol-3-phosphate dehydrogenase (NAD(P)+)
Topics	Bacterial Proteins (genetics) Bacteriophage lambda Culture Media (chemistry) Escherichia coli (genetics, growth & development, radiation effects, virology) Gene Expression Regulation, Bacterial Genes, Bacterial Glycerolphosphate Dehydrogenase (genetics) Phosphates Ultraviolet Rays

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