Distinct mechanisms for the endocytosis of muscarinic receptors and Gq/11.

Abstract

To determine whether the agonist-mediated endocytosis of muscarinic receptors and Gq/11 are mechanistically related events, the internalization of Gq/11 was monitored under conditions established to prevent muscarinic receptor endocytosis. Incubation of SH-SY5Y neuroblastoma cells with oxotremorine-M resulted in the translocation of both muscarinic receptors and Gq/11 into a 'light vesicle' membrane fraction. Although muscarinic receptor translocation was prevented by either the depletion of phosphoinositides or by disruption of clathrin assembly, the endocytosis of Gq/11 was unaffected. These results demonstrate that the agonist-induced internalization of muscarinic receptors and Gq/11 proceed via distinct mechanisms.

• Authors: S D Sorensen, D A Linseman, S K Fisher
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 372 Issue 3 Pg. 325-8 (May 21 1999) ISSN: 0014-2999 [Print] Netherlands
• PMID: 10395032 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Androstadienes
• Hypertonic Solutions
• Receptors, Muscarinic
• GTP-Binding Proteins
• Wortmannin

Topics

• Androstadienes (pharmacology)
• Animals
• Cells, Cultured
• Cricetinae
• Cricetulus
• Endocytosis (drug effects)
• GTP-Binding Proteins (metabolism)
• Hypertonic Solutions (pharmacology)
• Receptors, Muscarinic (metabolism)
• Wortmannin