Conservation of expression and N-terminal sequences of the Pasteurella haemolytica 31-kilodalton and Pasteurella trehalosi 29-kilodalton periplasmic iron-regulated proteins.

Abstract

This study examined the conservation of expression of a 31-kDa iron-regulated protein by serotypes of Pasteurella haemolytica and Pasteurella trehalosi associated with pasteurellosis of cattle and sheep. A polyclonal antibody prepared against the purified 31-kDa periplasmic iron-regulated protein from P. haemolytica serotype A1 showed that all P. haemolytica serotypes expressed similar 31-kDa proteins with identical N-terminal sequences, whereas P. trehalosi serotypes expressed immunologically different 29-kDa proteins with a different N-terminal sequence. Antibody to the 31-kDa iron-regulated protein was a useful tool to distinguish similarities and differences of the iron-regulated proteins of P. haemolytica and P. trehalosi.

• Authors: L B Tabatabai, G H Frank
• Journal: Clinical and diagnostic laboratory immunology (Clin Diagn Lab Immunol) Vol. 6 Issue 4 Pg. 617-20 (Jul 1999) ISSN: 1071-412X [Print] United States
• PMID: 10391874 (Publication Type: Journal Article)

Chemical References

• Bacterial Proteins
• Chlorides
• Ferric Compounds
• Lipoproteins
• Membrane Proteins
• Plp1 protein, Pasteurella haemolytica
• Sodium Dodecyl Sulfate
• Iron
• ferric chloride

Topics

• Amino Acid Sequence
• Animals
• Bacterial Proteins
• Cattle
• Chlorides
• Conserved Sequence
• Electrophoresis, Polyacrylamide Gel
• Ferric Compounds (pharmacology)
• Immunoblotting
• Iron (pharmacology)
• Lipoproteins (chemistry, genetics)
• Mannheimia haemolytica (chemistry)
• Membrane Proteins (chemistry, genetics)
• Osmotic Pressure
• Pasteurella (chemistry)
• Sodium Dodecyl Sulfate